Super resolution imaging of HER2 gene amplification

Masaya Okada; Takuya Kubo; Kanako Masumoto; Shigeki Iwanaga

doi:10.1117/12.2213918

12 April 2016 Super resolution imaging of HER2 gene amplification

Masaya Okada, Takuya Kubo, Kanako Masumoto, Shigeki Iwanaga

Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 97140E (2016) https://doi.org/10.1117/12.2213918
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.

Conference Presentation

Citation Download Citation

Masaya Okada, Takuya Kubo, Kanako Masumoto, and Shigeki Iwanaga "Super resolution imaging of HER2 gene amplification", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140E (12 April 2016); https://doi.org/10.1117/12.2213918

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