Dynamic focusing in the zebrafish beating heart

L. Andrés-Delgado; M. Peralta; N. Mercader; J. Ripoll

doi:10.1117/12.2211739

15 March 2016 Dynamic focusing in the zebrafish beating heart

L. Andrés-Delgado, M. Peralta, N. Mercader, J. Ripoll

Proceedings Volume 9717, Adaptive Optics and Wavefront Control for Biological Systems II; 971717 (2016) https://doi.org/10.1117/12.2211739
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Of the large amount of the animal models available for cardiac research, the zebrafish is extremely valuable due to its transparency during early stages of development. In this work a dual illumination laser sheet microscope with simultaneous dual camera imaging is used to image the beating heart at 200 fps, dynamically and selectively focusing inside the beating heart through the use of a tunable lens. This dual color dynamic focusing enables imaging with cellular resolution at unprecedented high frame rates, allowing 3D imaging of the whole beating heart of embryonic zebrafish.

Conference Presentation

Citation Download Citation

L. Andrés-Delgado, M. Peralta, N. Mercader, and J. Ripoll "Dynamic focusing in the zebrafish beating heart", Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971717 (15 March 2016); https://doi.org/10.1117/12.2211739

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