Analysis of erythrocyte flexibility by means of laser diffraction: effects of mechanical stress, photosensitization, and ozone

Rainer Bayer; Serdar Caglayan; Joerg G. Moser

doi:10.1117/12.148352

21 July 1993 Analysis of erythrocyte flexibility by means of laser diffraction: effects of mechanical stress, photosensitization, and ozone

Rainer Bayer, Serdar Caglayan, Joerg G. Moser

Author Affiliations +

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148352
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A newly developed system to determine red blood cell (RBC) flexibility is presented. It combines ektacytometry (laser diffraction) with image analysis. Laser light is passed through erythrocyte (RBC) suspensions which are sheared in a Searle like viscosimeter. The laser diffraction patterns are photographed by a linear CCD camera, and further analyzed by a computer. Flexibility is characterized by the quotient of minor and major axes of isointensity lines of the elliptically transformed diffraction patterns. The variation coefficient of the measurement is less than 1%. Exposing RBC to mechanical stress within the viscosimeter (shear rates from 130 to 2620/s, viscosity of the suspending medium 22 - 24 cpoise) is followed by a decrease of RBC flexibility. This effect is only detectable if elongation is measured at low shear rates (e.g., 260/s) and a threshold of stress (1100/s) is exceeded. It is not reversible and not accompanied with detectable hemolysis. Exposing whole blood to ozone (2 - 77 (mu) g/ml) does not alter RBC flexibility, but induces significant hemolysis at ozone > 7 (mu) g/ml. Thus the action of ozone can be regarded as an all or nothing effect either leaving the cell unaffected (flexibility) or destroying it (hemolysis).

Citation Download Citation

Rainer Bayer, Serdar Caglayan, and Joerg G. Moser "Analysis of erythrocyte flexibility by means of laser diffraction: effects of mechanical stress, photosensitization, and ozone", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148352

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available