Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Universal lab-on-a-chip platform for complex, perfused 3D cell cultures

[+] Author Affiliations
F. Sonntag, F. Schmieder, J. Ströbel, S. Grünzner, M. Busek, T. Steege, C. Polk, U. Klotzbach

Fraunhofer IWS Dresden (Germany)

K. Günther

TU Dresden (Germany)

Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970516 (March 21, 2016); doi:10.1117/12.2218606
Text Size: A A A
From Conference Volume 9705

  • Microfluidics, BioMEMS, and Medical Microsystems XIV
  • Bonnie L. Gray; Holger Becker
  • San Francisco, California, United States | February 13, 2016

abstract

The miniaturization, rapid prototyping and automation of lab-on-a-chip technology play nowadays a very important role. Lab-on-a-chip technology is successfully implemented not only for environmental analysis and medical diagnostics, but also as replacement of animals used for the testing of substances in the pharmaceutical and cosmetics industries. For that purpose the Fraunhofer IWS and partners developed a lab-on-a-chip platform for perfused cell-based assays in the last years, which includes different micropumps, valves, channels, reservoirs and customized cell culture modules. This technology is already implemented for the characterization of different human cell cultures and organoids, like skin, liver, endothelium, hair follicle and nephron. The advanced universal lab-on-a-chip platform for complex, perfused 3D cell cultures is divided into a multilayer basic chip with integrated micropump and application-specific 3D printed cell culture modules. Moreover a technology for surface modification of the printed cell culture modules by laser micro structuring and a complex and flexibly programmable controlling device based on an embedded Linux system was developed. A universal lab-on-a-chip platform with an optional oxygenator and a cell culture module for cubic scaffolds as well as first cell culture experiments within the cell culture device will be presented. The module is designed for direct interaction with robotic dispenser systems. This offers the opportunity to combine direct organ printing of cells and scaffolds with the microfluidic cell culture module. The characterization of the developed system was done by means of Micro-Particle Image Velocimetry (μPIV) and an optical oxygen measuring system. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

F. Sonntag ; F. Schmieder ; J. Ströbel ; S. Grünzner ; M. Busek, et al.
" Universal lab-on-a-chip platform for complex, perfused 3D cell cultures ", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970516 (March 21, 2016); doi:10.1117/12.2218606; http://dx.doi.org/10.1117/12.2218606


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.