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Proceedings Article

Imaging of anticancer agent distribution by a slit-scanning Raman microscope

[+] Author Affiliations
Yoshinori Harada, Dai Ping, Yoshihisa Yamaoka, Tetsuro Takamatsu

Kyoto Prefectural Univ. of Medicine (Japan)

Taisuke Ota, Keisaku Hamada, Katsumasa Fujita

Osaka Univ. (Japan)

Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685308 (February 08, 2008); doi:10.1117/12.763078
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From Conference Volume 6853

  • Biomedical Optical Spectroscopy
  • Anita Mahadevan-Jansen; Wolfgang Petrich; Robert R. Alfano; Alvin Katz
  • San Jose, CA | January 19, 2008

abstract

In recent years, various types of molecular imaging technologies have been developed, but many of them require probes and may have some influence on the distribution of the target molecules. In contrast, Raman microscopic analysis is effective for molecular identification of materials, and molecular imaging methods employing Raman scattering light can be applied to living organisms without use of any exogenous probes. Unfortunately, Raman microscopic imaging is rarely used in the biomedical field due to the weakness of Raman signals. When the conventional Raman microscopes are used, the acquisition of an image of a cell usually takes several hours. Recently, a slit-scanning confocal Raman microscope has been developed. It can acquire images of living cells and tissues with faster scanning speed. In this study, we used the slit-scanning confocal Raman microscope (RAMAN-11) to image the distribution of a drug in living cells. We could acquire images of the distribution of an anticancer reagent in living cells within several minutes. Since the wavelength of Raman scattering light is determined by the frequency of molecular vibration, the in situ mapping of the intracellular drugs without use of a probe is possible, suggesting that laser Raman imaging is a useful method for a variety of pharmacokinetic studies.

© (2008) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Yoshinori Harada ; Taisuke Ota ; Dai Ping ; Yoshihisa Yamaoka ; Keisaku Hamada, et al.
"Imaging of anticancer agent distribution by a slit-scanning Raman microscope", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685308 (February 08, 2008); doi:10.1117/12.763078; http://dx.doi.org/10.1117/12.763078


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