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In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with
the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of
anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a
molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion.
By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple
targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective
peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting
complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2
but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1
can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by
subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response
(maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective
targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also
reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging,
providing a better guidance for diagnosis and treatment of cancer.
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