Ultrasensitive detection of red blood cell lysing in a microfabricated semiconductor laser cavity

Paul Lee Gourley; T. French; Anthony E. McDonald; E. A. Shields; Mark F. Gourley M.D.

doi:10.1117/12.304380

26 March 1998 Ultrasensitive detection of red blood cell lysing in a microfabricated semiconductor laser cavity

Paul Lee Gourley, T. French, Anthony E. McDonald, E. A. Shields, Mark F. Gourley M.D.

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304380
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

In this paper we report investigations of semiconductor laser microcavities for use in detecting changes of human blood cells during lysing. By studying the spectral before and during mixing of blood fluids with di-ionized water, we are able to qualify the cell shape and concentration of hemoglobin in real time during the dynamical process of lysing. We find that the spectra can detect subtle changes that are orders of magnitude smaller than can be observed by standard optical microscopy. Such sensitivity in observing cell structural changes has implications for measuring cell fragility, monitoring apoptitic events in real time, development of photosensitizers for photodynamic therapy, and in-vitro micromanipulation techniques.

Citation Download Citation

Paul Lee Gourley, T. French, Anthony E. McDonald, E. A. Shields, and Mark F. Gourley M.D. "Ultrasensitive detection of red blood cell lysing in a microfabricated semiconductor laser cavity", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304380

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