The study of hydrogen peroxide level under cisplatin action using genetically encoded sensor hyper

A. S. Belova; A. G. Orlova; A. V. Maslennikova; A. A. Brilkina; I. V. Balalaeva; N. O. Antonova; N. M. Mishina; N. M. Shakhova; V. V. Belousov

doi:10.1117/12.2037737

7 March 2014 The study of hydrogen peroxide level under cisplatin action using genetically encoded sensor hyper

A. S. Belova, A. G. Orlova, A. V. Maslennikova, A. A. Brilkina, I. V. Balalaeva, N. O. Antonova, N. M. Mishina, N. M. Shakhova, V. V. Belousov

Author Affiliations +

Proceedings Volume 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI; 895612 (2014) https://doi.org/10.1117/12.2037737
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The aim of the work was to study the participation of hydrogen peroxide in reaction of cervical cancer cell line HeLa Kyoto on cisplatin action. Determination of hydrogen peroxide level was performed using genetically encoded fluorescent sensor HyPer2. The dependence of cell viability on cisplatin concentration was determined using MTT assay. Mechanisms of cell death as well as HyPer2 reaction was revealed by flow cytometry after 6-hours of incubation with cisplatin in different concentrations. Cisplatin used in low concentrations had no effect on hydrogen peroxide level in HeLa Kyoto cells. Increase of HyPer2 fluorescence was detected only after exposure with cisplatin in high concentration. The reaction was not the consequence of cell death.

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A. S. Belova, A. G. Orlova, A. V. Maslennikova, A. A. Brilkina, I. V. Balalaeva, N. O. Antonova, N. M. Mishina, N. M. Shakhova, and V. V. Belousov "The study of hydrogen peroxide level under cisplatin action using genetically encoded sensor hyper", Proc. SPIE 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI, 895612 (7 March 2014); https://doi.org/10.1117/12.2037737

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