Mr. Kang Du Profile

Kang Du

at Changchun Univ of Science and Technology

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Proceedings Article | 15 December 2022 Paper

Determination of glucose solution concentration by laser confocal Raman spectroscopy

Kang Du, Yangyang Hua, Tingting Wang, Meng Zhao, Hongxing Cai

Proceedings Volume 12478, 124780P (2022) https://doi.org/10.1117/12.2651141

KEYWORDS: Glucose, Raman spectroscopy, Laser spectroscopy, Confocal microscopy, Water, Blood, Spectroscopy, Quantitative analysis, Objectives, Nondestructive evaluation

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In this paper, the laser confocal Raman spectrum of glucose standard solution was measured and studied. The relative intensity of the characteristic peak of glucose and the characteristic peak of water in the solution was used as the ratio of the measured content to measure the concentration of glucose solution.Put the prepared glucose solutions of different concentrations in a cuvette, place them on a glass slide, and place them under the field of view of the objective lens of the laser microscope Raman spectrometer stage, adjust the focal length through the eyepiece, focus the laser on the surface of the solution, and then measure Raman spectra of different solutions.The experimental results show that the relative intensity of the 1125cm^-1 peak has the same trend as the actual value of glucose concentration, and there is a good linear relationship between them. This linear correlation reaches more than 0.93, and the measured LOD value is 160mg/dL This result confirms that the glucose concentration detected by Raman spectroscopy using this analytical method can be close to the normal value of human blood sugar. This will help to explore the relationship between the ratio of blood glucose peak to hemoglobin peak intensity and blood glucose content, and lay a good foundation for noninvasive blood glucose research.

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