While artificial cultivation is gaining prominence due to rapid climate change, lighting costs remain a challenge. Therefore, research is needed to cultivate plants more efficiently. At the same time, it has been found that a mixture of far-red light (FR) and red light (R) also promotes growth through the action of phytochrome, a photoreceptor in the plant body. However, these studies require time and damaging of the plant to measure the dry mass and area of leaves, and immediate effects have not been investigated so far. Therefore, in this study, we propose laser biospeckle to evaluate the relationship between plant growth duration and FR response. Laser biospeckles are formed by light scattered from organelles in plant tissues by laser irradiation. The intensity of these speckles changes with time, and by examining these changes, the activity inside the plant can be evaluated. Biospeckles of arugula leaves were obtained by irradiating the leaves with laser light of wavelength 852 nm and simultaneously with LED light of wavelength 735 nm (FR) and 630 nm (R). Biospeckle movies under FR and non-irradiation were recorded by a CMOS camera at 15 frames/sec for 20 seconds. From the movie, correlation between the first frame and the subsequent frames were calculated. Experimental results showed that arugula at 1-month after seed planting showed an immediate FR response, while those at 3-month showed a sustained response. The relationship between biospeckle movement and plant growth behaviour is under investigation.
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