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Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles
on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the
loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold
substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide
decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized
particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides
and gold nanoparticles, particle-peptide hybrid multi layers could be formed.
Daniel Aili,Hsu Shu Han,Karin Enander,Lars Baltzer, andBo Liedberg
"Controlled assembly of gold nanoparticles using De Novo designed polypeptide scaffolds", Proc. SPIE 6885, MEMS/MOEMS Components and Their Applications V. Special Focus Topics: Transducers at the Micro-Nano Interface, 688506 (20 February 2008); https://doi.org/10.1117/12.775806
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Daniel Aili, Hsu Shu Han, Karin Enander, Lars Baltzer, Bo Liedberg, "Controlled assembly of gold nanoparticles using De Novo designed polypeptide scaffolds," Proc. SPIE 6885, MEMS/MOEMS Components and Their Applications V. Special Focus Topics: Transducers at the Micro-Nano Interface, 688506 (20 February 2008); https://doi.org/10.1117/12.775806