The toolbox of fluorescence standards: flexible calibration tools for the standardization of fluorescence-based measurements

Ute Resch-Genger; K. Hoffmann; C. Würth; T. Behnke; A. Hoffmann; D. Pfeifer; A. Engel

doi:10.1117/12.853133

5 May 2010 The toolbox of fluorescence standards: flexible calibration tools for the standardization of fluorescence-based measurements

Ute Resch-Genger, K. Hoffmann, C. Würth, T. Behnke, A. Hoffmann, D. Pfeifer, A. Engel

Author Affiliations +

Proceedings Volume 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX; 76661J (2010) https://doi.org/10.1117/12.853133
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

To improve the reliability of fluorescence data in the life and material sciences and to enable accreditation of fluorescence techniques, standardization concepts are required that guarantee and improve the comparability of fluorescence measurements. At the core of such concepts are simple and evaluated fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and for instrument performance validation (IPV). Similarly in need are fluorescence intensity standards for the quantification from measured intensities and for signal referencing, thereby accounting for excitation light-induced intensity fluctuations. These standards should be preferably certified, especially for use in regulated areas like medical diagnostics. This encouraged us to develop liquid and solid standards for different fluorescence parameters and techniques for use under routine measurement conditions in different formates. Special emphasis was dedicated to the determination and control of the spectral responsivity of detection systems, wavelength accuracy, homogeneity of illumination, and intensity referencing for e.g. spectrofluorometers, fluorescence sensors and confocal laser scanning fluorescence microscopes. Here, we will present design concepts and examples for mono- and multifunctional fluorescence standards that provide traceability to radiometric units and present a first step towards a toolbox of standards.

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Ute Resch-Genger, K. Hoffmann, C. Würth, T. Behnke, A. Hoffmann, D. Pfeifer, and A. Engel "The toolbox of fluorescence standards: flexible calibration tools for the standardization of fluorescence-based measurements", Proc. SPIE 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX, 76661J (5 May 2010); https://doi.org/10.1117/12.853133

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