Philip J. R. Roche,1 Maurice C-K. Cheung,1 Mohamed Najih,1 Laura-Isobel McCall,2 Ibrahim Fakih,1 Vamsy P. Chodavarapu,1 Brian Ward,2 Momar Ndao,2 Andrew G. Kirk1
1McGill Univ. (Canada) 2Montreal General Hospital (Canada)
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Visceral leishmaniasis (L.donovani) is a protozoan infection that attacks mononuclear phagocytes and causes the liver
and spleen damage that can cause death. The investigation presented is a proof of concept development applying a
plasmonic diagnostic platform with simple microfluidic sample delivery and optical readout. An immune-assay method
is applied to the quantification of A2 protein, a highly immunogenic biomarker for the pathogen. Quantification of A2
was performed in the ng/ml range, analysis by ELISA suggested that a limit of 0.1ng/ml of A2 is approximate to 1
pathogen per ml and the sensing system shows the potential to deliver a similar level of quantification. Significant
reduction in assay complexity as further enzyme linked enhancement is not required when applying a plasmonic methodology to an immunoassay. The basic instrumentation required for a portable device and potential dual optical readout where both plasmonic and photoluminescent response are assessed and investigated including consideration of the application of the device to testing where non-literate communication of results is considered and issues of
performance are addressed.
Philip J. R. Roche,Maurice C-K. Cheung,Mohamed Najih,Laura-Isobel McCall,Ibrahim Fakih,Vamsy P. Chodavarapu,Brian Ward,Momar Ndao, andAndrew G. Kirk
"A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290C (14 February 2012); https://doi.org/10.1117/12.910146
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Philip J. R. Roche, Maurice C-K. Cheung, Mohamed Najih, Laura-Isobel McCall, Ibrahim Fakih, Vamsy P. Chodavarapu, Brian Ward, Momar Ndao, Andrew G. Kirk, "A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani," Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290C (14 February 2012); https://doi.org/10.1117/12.910146