Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension
(Conference Presentation)

Natan T. Shaked

doi:10.1117/12.2209306

27 April 2016 Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension (Conference Presentation)

Natan T. Shaked

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 971804 (2016) https://doi.org/10.1117/12.2209306
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Our latest methods for non-invasive label-free acquisition of the three-dimensional (3-D) refractive-index maps of live cells in suspension are reviewed. These methods are based on the acquisition of off-axis interferograms of single or multiple cells in suspension from different angles using an external interferometric module, while fully rotating each cell using micro-manipulations. The interferometric projections are processed via computed tomographic phase microscopy reconstruction technique, which considers optical diffraction effects, into the 3-D refractive-index structure of the suspended cell. Till now, tomographic phase microscopy was obtained by acquiring a series of interferograms of the light transmitted through the sample in different angles by either using an entire sample rotation, or patch clamping a single cell, which is invasive to the cells, or alternatively, using various angles of illumination, which causes a limited acceptance angle, and an incomplete 3-D Fourier spectrum. In contrast, our methods allow fast acquisition with full angular range, and thus obtain an accurate 3-D refractive-index map of the imaged cell. By inspection of the 3-D refractive-index distribution of cells in suspension, the proposed methods can be useful for high-throughput, label-free characterization of biological processes and cellular transformations from healthy to pathological conditions.

Conference Presentation

Citation Download Citation

Natan T. Shaked "Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 971804 (27 April 2016); https://doi.org/10.1117/12.2209306

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

3D acquisition

Interferometry

Microscopy

Tomography

3D image processing

Diffraction

Inspection

Show All Keywords

Keywords/Phrases

Search In:

Publication Years