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Herein, we will use Macroscopy Fluorescence Lifetime Forster Resonance Energy Transfer (MFLI FRET) to quantitatively image in vivo and non-invasively the efficacy of receptor-targeted drug delivery systems. We have established an antibody-to-target imaging pipeline to develop novel near-infrared (NIR)-labeled probes to interrogate and quantify the dynamics of drug-receptor binding in HER2 breast cancer tumor xenografts.
Amit Verma,Catherine Sherry,Nanxue Yuan,Vikas Pandey,John Williams,Xavier Intes, andMargarida M. Barroso
"Using meditope-based antibody labeling to improve fluorescence lifetime FRET imaging", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470S (13 March 2024); https://doi.org/10.1117/12.3008753
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Amit Verma, Catherine Sherry, Nanxue Yuan, Vikas Pandey, John Williams, Xavier Intes, Margarida M. Barroso, "Using meditope-based antibody labeling to improve fluorescence lifetime FRET imaging," Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470S (13 March 2024); https://doi.org/10.1117/12.3008753