Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array

Liang Song; Konstantin I. Maslov; Rachel Bitton; K. Kirk Shung; Lihong V. Wang

doi:10.1117/1.2976141

1 September 2008 Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array

Liang Song, Konstantin I. Maslov, Rachel Bitton, K. Kirk Shung, Lihong V. Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 13, Issue 5, 054028 (September 2008). https://doi.org/10.1117/1.2976141

Abstract

We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50 Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1 Hz with postbeamforming. To our knowledge, this speed is currently the fastest in photoacoustic imaging. A custom-designed light delivery system is integrated with a 30-MHz ultrasound linear array to realize dark-field reflection-mode imaging. Linear mechanical scanning of the array produces 3-D images. The system has axial, lateral, and elevational resolutions of 25, 70, and 200 μm, respectively, and can image 3 mm deep in scattering biological tissues. Volumetric images of subcutaneous vasculature in rats are demonstrated in vivo. Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Liang Song, Konstantin I. Maslov, Rachel Bitton, K. Kirk Shung, and Lihong V. Wang "Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array," Journal of Biomedical Optics 13(5), 054028 (1 September 2008). https://doi.org/10.1117/1.2976141

Published: 1 September 2008

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Cited by 74 scholarly publications and 18 patents.

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KEYWORDS

Imaging systems

Ultrasonography

Photoacoustic microscopy

3D image processing

Photoacoustic imaging

3D photoacoustic microscopy

In vivo imaging

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