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14 September 2012 Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy
Thomas Bruns, Sarah Schickinger, Rainer Wittig, Herbert Schneckenburger
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Journal of Biomedical Optics, Vol. 17, Issue 10, 101518 (September 2012). https://doi.org/10.1117/1.JBO.17.10.101518
Abstract
A device for selective plane illumination microscopy (SPIM) of three-dimensional multicellular spheroids, in culture medium under stationary or microfluidic conditions, is described. Cell spheroids are located in a micro-capillary and a light sheet, for illumination, is generated in an optical setup adapted to a conventional inverse microscope. Layers of the sample, of about 10 μm or less in diameter, are, thus, illuminated selectively and imaged by high resolution fluorescence microscopy. SPIM is operated at low light exposure even if a larger number of layers is imaged and is easily combined with laser scanning microscopy. Chinese hamster ovary cells expressing a membrane-associated green fluorescent protein are used for preliminary tests, and the uptake of the fluorescent marker, acridine orange via a microfluidic system, is visualized to demonstrate its potential in cancer research such as for the detection of cellular responses to anticancer drugs.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Thomas Bruns, Sarah Schickinger, Rainer Wittig, and Herbert Schneckenburger "Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy," Journal of Biomedical Optics 17(10), 101518 (14 September 2012). https://doi.org/10.1117/1.JBO.17.10.101518
Published: 14 September 2012
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Cited by 40 scholarly publications and 6 patents.
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KEYWORDS
Luminescence
Microscopy
Microscopes
Objectives
Microfluidics
Capillaries
Confocal microscopy
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