Laser-induced fluorescence of collagen and cholestrol

N. Moise; C. Carp; Mihail-Lucian Pascu

doi:10.1117/12.203579

8 March 1995 Laser-induced fluorescence of collagen and cholestrol

N. Moise, C. Carp, Mihail-Lucian Pascu

Proceedings Volume 2461, ROMOPTO '94: Fourth Conference in Optics; (1995) https://doi.org/10.1117/12.203579
Event: ROMOPTO '94: 4th Conference on Optics, 1994, Bucharest, Romania

Abstract

In this paper, laser induced fluorescence (LIF) and fluorescence lifetime of collagen and cholesterol solutions when excited with 337.1 nm radiation emitted by subnanosecond (700 psec) nitrogen pulsed laser are reported: absorption and excitation spectra of these biomolecules were obtained, too. The fluorescence excitation of collagen and cholesterol solutions was performed focusing the laser radiation on a CeramOptec quartz optical fiber with a core of 1000 micrometers . The fluorescence emission was collected using quartz optical fiber with a core of 800 micrometers . The collagen fluorescence spectra, at concentrations between 0.1 mg/ml and 5.0 mg/ml in acetic acid of initial concentrations 0.01%, 0.1% and 1%, respectively, in distilled water, exhibit a peak at 415 nm with FWHM of about 100 nm; the cholesterol fluorescence spectra, at concentrations from 0.1 mg/ml to 5.0 mg/ml in isopropyl alcohol, have a peak at 395 nm and FWHM of 60 nm. The higher values of LIF signals for cholesterol are due to the higher quantum yield. The measurements of LIF lifetimes were performed using a fast PIN photodiode with a rise time of about 200 psec. For collagen solutions a fluorescence lifetime of 5 nsec was obtained; for cholesterol solutions the measured fluorescence lifetime was 6 nsec. The overall errors in fluorescence lifetime measurements was 6%.

Citation Download Citation

N. Moise, C. Carp, and Mihail-Lucian Pascu "Laser-induced fluorescence of collagen and cholestrol", Proc. SPIE 2461, ROMOPTO '94: Fourth Conference in Optics, (8 March 1995); https://doi.org/10.1117/12.203579

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