In this work, we evaluated the acoustic and optical properties of silk protein-based hydrogels to investigate its potential as a phantom material. Acoustic properties include the speed of sound and acoustic attenuation of silk scaffolds at various concentrations. Optical properties include optical absorption and reduced scattering measured between 400 nm to 1200 nm to coincide with common photoacoustic imaging bandwidths. The results indicate that silk is an acceptable phantom material for ultrasound and photoacoustic systems as it inherently displays similar acoustic properties and reduced scattering as various tissue types while displaying low absorption.
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