Dipicolinic acid (DPA), bound to calcium (Ca), is a main component of bacterial endospores. Complexation of DPA with lanthanide ions, particularly Terbium (Tb), allows for rapid detection via monitoring the lanthanide luminescence, with applications spanning from cell imaging to contamination and biohazard detection, to sterilization control. Here we present time-resolved luminescence of the Tb-DPA complex upon UV excitation at 266 nm. Our measurements directly monitor the luminescence dynamics and speak for a rise of the luminescence on the ns time scale, which is orders of magnitude faster than previously reported, and raise questions about the details of the energy transfer process in this complex and the states involved. The results are relevant for the design of more sensitive detection schemes for Tb-DPA fluorescence, as well as for the design of novel Tb-based luminescence probes or novel fluorescence probes working as FRET acceptors of Tb energy.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.