SPR method was applied for studding the herpes simplex virus replication kinetic in human cells and the inhibitory effect of acyclovir. The obtained results were compared with classical endpoint and quantification techniques
Random lasers are a novel disorder-based laser light source with many photonics applications. In the present work we study the optical properties of a new unordered medium consisted of an epoxy resin as matrix with dissolved different binary solutions of Rhodamine 6G and Rhodamine B. The choice of these dyes is related to their great application in laser physics. A detailed study of the spectral dependence of fluorescence is performed. The spontaneous emission is very intensive and follows the spectral dependence of dyes transmission and exceeds 750 nm. The threshold of the laser medium is 1.6 mJ for 10 ns pulse at second harmonics of Nd:YAG laser, and full width at half maximum (FWHM) is around 1.5 nm. Slope efficiency of the medium is 16%. The laser generation without resonator was studied also and lasing emission at 597 nm was observed. The fact can be explained by random lasing provided by the medium. This is in good relation and in accordance with the intensive scattering inherent to the medium which is registered. Not used additional scatter centers such as ZnO nanorods or Ag, Al, Al2O3, TiO2 agglomerates and others in our experiments.
Apart from the huge physiological importance of hemo-, myo- globin and hemin, they play also an important role as robust and effective recognition agents in chemical and biological sensors. In this aspect the key factor in developing a reliable biosensor is the immobilization on the transducer. Usually immobilization can be produced by chemical methods, but there is frequently a need for homogeneous films of well-controlled thickness or films which can be deposited in a dry environment. The film thickness is of main importance for the optical transducer detected by Surface Plasmon Resonance (SPR) what is used in our study. Hundreds of immobilization protocols have been developed in an effort to ensure high performance sensing. All of them are focused on finding and deposition of appropriate matrices in which the recognition medium can be incorporated. However, the matrix always deteriorates the effectiveness of recognition. It seems that the best approach is to perform direct immobilization of the recognition medium. However, this is not always possible regarding the organic materials – the problem is whether the deposition retains the bioactivity of the recognition agent. On the other hand, the type of the transducer also imposes constrains. For example, the direct immobilization of the proteins is not possible for electrochemical sensors, because of the distance between the redox center and electrodes is too long. Evaluating the pros and cons of organic (protein) film deposition we have considered to study the possibility for direct immobilization of myoglobin, hemoglobin and hemin on SPR transducer. To best of our knowledge, SPR biochip with immobilized myoglobin, hemoglobin and hemin has never been constructed before. We have used spin coating, for direct immobilization and matrix-assisted pulsed-laser evaporation (MAPLE) for elaboration of the SPR biochip. The performance of both SPR chips – direct and MAPLE immobilized, was studied by SPR registration of the binding activity of myo- and hemo- globin ligands with carbon monoxide (CO), carbon dioxide (CO2) and nitride oxide (NO).
Surface plasmon resonance (SPR) is widely applied for bio/ chemical sensing. The main problem for all sensors is accuracy and sensitivity enhancement. For SPR sensors the accuracy depends on the characteristics of the plasmon resonance. In this work, we study SPR excitation assisted by liquid crystal layer. We consider Kretshmann configuration where a liquid crystal is sandwiched between a prism and glass plate, while the gold layer is evaporated over the glass plate. We show that the Bragg reflection of cholesteric liquid crystals modifies the plasmon resonance in a way increasing the accuracy of its location. Also, features of plasmon resonance excitation are studied for chiral liquid crystals.
Optical vortices have been applied for the purpose of polarimetry measurement. Vortex polarimetry combines the features of null polarimetry and imaging Fourier polarimetry providing one shot measurement. Different polarimeter systems and limiting accuracy factors are discussed.
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