Measurements of human milk fat content are essential for lactation care and research. We propose to quantify milk fat in non-homogenized human milk based on angular light scattering. Therefore, we measured the angular scattering profiles of milk from five donors using a goniometric light scattering setup. We also measured the milk fat globule (MFG) size distributions with 3D confocal laser scanning microscopy and use this as input in a Monte Carlo simulation. Both experimental and simulated angular scattering profiles are strongly dependent on fat concentration. The effect of the MFG size distribution on the scattering profiles will be discussed.
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