Mr. Haodong Wu Profile

Haodong Wu

at Nanjing Univ

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SPIE Journal Paper | 8 April 2023 Open Access

Multiplexed optical fiber cell temperature sensing system with high sensitivity and accuracy

Yu Guo, Yanxia Shen, Xinyu Sun, Shulin Song, Haodong Wu, Xiulan Sun

JBO, Vol. 28, Issue 04, 047001, (April 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.4.047001

KEYWORDS: Fiber lasers, Sensing systems, Fiber Bragg gratings, Temperature metrology, Laser frequency, Sensors, Glasses, Optical fibers, Multiplexing, Resistance

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SignificanceA multiplexed fiber laser sensing system for cell temperature is proposed. To the best of the authors’ knowledge, this is the first multilongitudinal mode (MLM) optical fiber laser sensor array designed for cell temperature sensing.AimA two-channel cell temperature sensing system with high sensitivity and real-time sensing capability is achieved. The temperature change of human hepatoellular carcinomas (HepG2) cells under the influence of exogenous chemical aflatoxin B1 (AFB1) can be monitored in real time.ApproachA fiber laser cavity consists of a pair of fiber Bragg gratings (FBGs) with matched central wavelengths and a piece of erbium-doped fiber (EDF). The static FBG is utilized for design of fiber laser cavity and laser modes selection. In comparison, the sensing FBG is used for cell temperature sensing. The sensing FBG has a length of 10 mm and a diameter of 200 μm. Beat frequency signals (BFS) are generated by MLM lasers after optical-to-electrical conversion at a photodetector. Frequency change of a BFS is closely related to the reflected wavelength change of the sensing FBG. Through frequency division multiplexing, two fiber laser cavities are designed in the sensing system for two-channel temperature sensing. Frequency shift of a BFS that represents temperature change of cells can be automatically recorded in seconds.ResultsA two-channel cell temperature sensing system is designed with high sensitivities of 101.62 and 119.82 kHz / ° C, respectively. The temperature change of HepG2 cells under the influence of exogenous chemical AFB1 is monitored in real time.ConclusionsThe proposed system has the advantages of simple structure, high sensitivity, and two-channel sensing capability. Our study provides a simple and effective method to design a fiber laser sensor system without complex demodulation techniques and expensive optical components.

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