Mr. Hubertus Hakert Profile

Hubertus Hakert

Intern/Student Rsrch Assistant at Univ zu Luebeck

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Publications (7)

Proceedings Article | 8 March 2023 Presentation + Paper

900 nm swept source FDML laser with kW peak power

Philipp Lamminger, Hubertus Hakert, Simon Lotz, Jan Philip Kolb, Tonio Kutscher, Sebastian Karpf, Robert Huber

Proceedings Volume 12400, 124001I (2023) https://doi.org/10.1117/12.2649663

KEYWORDS: Fiber lasers, Raman spectroscopy, Microscopy, Tunable filters, Photonic crystal fibers, Four wave mixing, Wavelength tuning

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Proceedings Article | 4 March 2019 Presentation

Label-free imaging of tumorous tissue in the Raman fingerprint region with time-encoded (TICO) stimulated Raman scattering (Conference Presentation)

Hubertus Hakert, Matthias Eibl, Sebastian Karpf, Barbara Wollenberg, Ralph Pries, Robert Huber

Proceedings Volume 10882, 108821R (2019) https://doi.org/10.1117/12.2509851

KEYWORDS: Raman spectroscopy, Tissues, Tumors, Raman scattering, Microscopy, Fiber lasers, Signal to noise ratio, Nonlinear optics, Proteins, Biopsy

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Proceedings Article | 22 February 2019 Paper

Virtual HE histology by fiber-based picosecond two-photon microscopy

Jan Philip Kolb, Daniel Weng, Hubertus Hakert, Matthias Eibl, Wolfgang Draxinger, Tobias Meyer, Thomas Gottschall, Ralf Brinkmann, Reginald Birngruber, Jürgen Popp, Jens Limpert, Sebastian Nino Karpf, Robert Huber

Proceedings Volume 10882, 108822F (2019) https://doi.org/10.1117/12.2507866

KEYWORDS: Luminescence, Femtosecond phenomena, Picosecond phenomena, Fiber lasers, Optical filters, Tissues, Laser systems engineering, Objectives, Microscopes

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Proceedings Article | 28 July 2017 Paper

Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Tom Pfeiffer, Jan Philip Kolb, Robert Huber

Proceedings Volume 10414, 1041403 (2017) https://doi.org/10.1117/12.2286035

KEYWORDS: Two photon excitation microscopy, FRET and FLIM microscopy, Fiber coupled lasers, Microscopy, Fiber lasers, Fluorescence lifetime imaging, Luminescence, Fiber optics, Nonlinear optics

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Proceedings Article | 28 July 2017 Paper

Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition

Hubertus Hakert, Matthias Eibl, Sebastian Karpf, Robert Huber

Proceedings Volume 10414, 1041408 (2017) https://doi.org/10.1117/12.2287947

KEYWORDS: Raman scattering, Microscopy, Fiber lasers, Image acquisition, Nonlinear optics, Fiber optics, Raman spectroscopy, Mode locking

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Modern biomedical imaging modalities aim to provide researchers a multimodal contrast for a deeper insight into a specimen under investigation. A very promising technique is stimulated Raman scattering (SRS) microscopy, which can unveil the chemical composition of a sample with a very high specificity. Although the signal intensities are enhanced manifold to achieve a faster acquisition of images if compared to standard Raman microscopy, there is a trade-off between specificity and acquisition speed. Commonly used SRS concepts either probe only very few Raman transitions as the tuning of the applied laser sources is complicated or record whole spectra with a spectrometer based setup. While the first approach is fast, it reduces the specificity and the spectrometer approach records whole spectra -with energy differences where no Raman information is present-, which limits the acquisition speed. Therefore, we present a new approach based on the TICO-Raman concept, which we call sparse-sampling. The TICO-sparse-sampling setup is fully electronically controllable and allows probing of only the characteristic peaks of a Raman spectrum instead of always acquiring a whole spectrum. By reducing the spectral points to the relevant peaks, the acquisition time can be greatly reduced compared to a uniformly, equidistantly sampled Raman spectrum while the specificity and the signal to noise ratio (SNR) are maintained. Furthermore, all laser sources are completely fiber based. The synchronized detection enables a full resolution of the Raman signal, whereas the analogue and digital balancing allows shot noise limited detection. First imaging results with polystyrene (PS) and polymethylmethacrylate (PMMA) beads confirm the advantages of TICO sparse-sampling. We achieved a pixel dwell time as low as 35 μs for an image differentiating both species. The mechanical properties of the applied voice coil stage for scanning the sample currently limits even faster acquisition.

Proceedings Article | 7 March 2017 Presentation + Paper

Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Torben Blömker, Robert Huber

Proceedings Volume 10083, 100831C (2017) https://doi.org/10.1117/12.2251965

KEYWORDS: Fiber amplifiers, Fiber lasers, Two photon imaging, Semiconductor lasers, Raman scattering, Laser sources, Diodes, Amplifiers, Modulation, Switching, Raman spectroscopy, Picosecond phenomena, Switches, Four wave mixing

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Proceedings Article | 21 February 2017 Presentation + Paper

Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Robert Huber

Proceedings Volume 10069, 100691F (2017) https://doi.org/10.1117/12.2250831

KEYWORDS: Fiber lasers, Fiber amplifiers, Semiconductor lasers, Two photon excitation microscopy, Laser sources, Luminescence, Two photon imaging, Microscopy, Fluorescence lifetime imaging, Analog electronics, Signal detection, Photons, Data acquisition, Picosecond phenomena, Microscopes

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Showing 5 of 7 publications

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