Ms. Jocelyn Martinez Profile

Jocelyn Martinez

at Texas A&M University

SPIE Involvement:

Author | Student Chapter Treasurer

Publications (4)

SPIE Journal Paper | 19 December 2024 Open Access

Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism

Anna Theodossiou, Jocelyn Martinez, Alex Walsh

JBO, Vol. 29, Issue 12, 126501, (December 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.12.126501

KEYWORDS: Light sources and illumination, Cameras, Autofluorescence, Autofluorescence imaging, Mode conditioning cables, Fluorescence, Cyanide, Fluorescence imaging, Microscopes, Photobleaching

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SignificanceCellular metabolic dynamics can occur within milliseconds, yet there are no optimal tools to spatially and temporally capture these events. Autofluorescence imaging can provide metabolic information on the cellular level due to the intrinsic fluorescence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD).AimOur goal is to build and evaluate a widefield microscope optimized for rapid autofluorescence imaging of metabolic changes in cells.ApproachA widefield, fluorescence microscope was assembled from an inverted microscope base, an light-emitting diode (LED) for excitation, and an image splitter for simultaneous but separate imaging of two bandwidths of emission (451/106 and 560/94 nm) on a single scientific complementary metal–oxide–semiconductor (sCMOS) camera. MCF-7 cells and primary murine hippocampal neurons were metabolically perturbed using cyanide and imaged to optimize illumination and camera exposure. To capture a rapid change in metabolism, MCF-7 cells were starved for 1 h and imaged while reintroduced to glucose.ResultsSignificant differences in the optical redox ratio (ORR) and intensity of NAD(P)H divided by the summed intensities of NAD(P)H and FAD were quantified for cyanide-treated neurons and MCF-7 cells at illumination powers above 0.30 mW and camera exposures as low as 5 ms; however, low illumination and camera exposures hindered the ability to identify subcellular features. Minimal photobleaching was quantified for 30 s of continuous imaging for illuminations at 4.14 mW and below. Using the optimized illumination power of 4.14 mW and an exposure of 10 ms, continuous autofluorescence imaging of starved MCF-7 cells demonstrated a rapid, yet heterogeneous, increase in the ORR of cells upon exposure to glucose.ConclusionsUltimately, this widefield autofluorescence imaging system allowed for dynamic imaging and quantification of cellular metabolism at 99.6 Hz.

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Proceedings Article | 7 February 2023 Presentation

Infrared light effects on coenzyme binding and cellular metabolism

Jocelyn Martinez, Anna Theodossiou, Alex Walsh

Proceedings Volume PC11958, PC1195807 (2023) https://doi.org/10.1117/12.2608239

KEYWORDS: Infrared radiation, Mode conditioning cables, Infrared imaging, Short wave infrared radiation, Thermal modeling, Neurons, Infrared technology, Thermography, Nerve, Ions

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Proceedings Article | 2 March 2022 Presentation

High speed autofluorescence imaging of the effects of short pulses of infrared light

Alex Walsh, Jocelyn Martinez, Anna Theodossiou

Proceedings Volume PC11971, PC119710B (2022) https://doi.org/10.1117/12.2613561

KEYWORDS: Infrared radiation, Infrared imaging, Auto-fluorescence imaging, Short wave infrared radiation, Thermal modeling, Neurons, Mode conditioning cables, Microscopy, Machine learning, Luminescence

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Proceedings Article | 5 March 2021 Presentation

Fluorescence lifetime imaging of metabolic co-enzymes to determine cell type and function

Alex Walsh, Linghao Hu, Elizabeth Cardona, Nianchao Wang, Jocelyn Martinez, Uyen Nguyen, Naman Bala

Proceedings Volume 11648, 116480K (2021) https://doi.org/10.1117/12.2577866

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Tumors, Tissues, Multiphoton fluorescence microscopy, Mode conditioning cables, Image segmentation, Image classification, Image analysis, Cancer

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