Dr. Joseph R. Lakowicz Profile

Dr. Joseph R. Lakowicz

Professor and Director at Univ of Maryland School of Medicine

SPIE Involvement:

Conference Program Committee | Track Chair | Editorial Board Member: Journal of Biomedical Optics | Editor | Author

Publications (105)

SPIE Journal Paper | 17 May 2018 Open Access

Glucose-sensitive silicone hydrogel contact lens toward tear glucose monitoring

Ramachandram Badugu, Edward Albert Reece, Joseph Lakowicz

JBO, Vol. 23, Issue 05, 057005, (May 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.5.057005

KEYWORDS: Glucose, Contact lenses, Silicon, Blood, Luminescence, Sensors, Polymers, Eye, Water, Oxygen

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Proceedings Article | 12 February 2018 Presentation + Paper

Towards early detection of age-related macular degeneration with tetracyclines and FLIM

Henryk Szmacinski, Kavita Hegde, Hui-Hui Zeng, Katayoun Eslami, Adam Puche, Joseph Lakowicz, Imre Lengyel, Richard Thompson

Proceedings Volume 10484, 1048409 (2018) https://doi.org/10.1117/12.2303768

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Retina, Modulation, Microscopy, Eye, In vivo imaging, Data modeling

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SPIE Journal Paper | 25 April 2014 Open Access

Application of phasor plot and autofluorescence correction for study of heterogeneous cell population

Henryk Szmacinski, Vladimir Toshchakov, Joseph Lakowicz

JBO, Vol. 19, Issue 04, 046017, (April 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.4.046017

KEYWORDS: Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Proteins, Luminescence, Modulation, Data corrections, Data modeling, Molecules, Fluorescent proteins, Receptors

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Proceedings Article | 6 February 2012 Paper

Plasmon-controlled fluorescence and single DNA strand sequencing

Nuriye Akbay, Krishanu Ray, Mustafa Chowdhury, Joseph Lakowicz

Proceedings Volume 8234, 82340M (2012) https://doi.org/10.1117/12.916177

KEYWORDS: Luminescence, Aluminum, Nanoparticles, Platinum, Quartz, Finite-difference time-domain method, Nanostructures, Metals, Chemical mechanical planarization, Ultraviolet radiation

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Proceedings Article | 6 February 2012 Paper

Distance-dependent intrinsic fluorescence of proteins on aluminum nanostructures

Nuriye Akbay, Joseph Lakowicz, Krishanu Ray

Proceedings Volume 8234, 823417 (2012) https://doi.org/10.1117/12.928379

KEYWORDS: Luminescence, Aluminum, Proteins, Quartz, Nanostructures, Particles, Metals, Mie scattering, Nanolithography, Nanoparticles

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SPIE Journal Paper | 1 November 2011 Open Access

Target molecule imaging on tissue specimens by fluorescent metal nanoprobes

Jian Zhang, Yi Fu, Joseph Lakowicz, Xuehong Xu

JBO, Vol. 16, Issue 11, 116004, (November 2011) https://doi.org/10.1117/12.10.1117/1.3644394

KEYWORDS: Tissues, Metals, Molecules, Nanoprobes, Luminescence, Target detection, Silica, Silver, Optical spheres, Tissue optics

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SPIE Journal Paper | 1 January 2011 Open Access

Detection of CXCR4 receptors on cell surface using a fluorescent metal nanoshell

Jian Zhang, Yi Fu, Richard Zhao, Joseph Lakowicz

JBO, Vol. 16, Issue 1, 016011, (January 2011) https://doi.org/10.1117/12.10.1117/1.3528623

KEYWORDS: Metals, Receptors, Molecules, Luminescence, Silica, Confocal microscopy, Image analysis, Medicine, Molecular imaging, Near field

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Proceedings Article | 3 March 2010 Paper

The use of aluminum nanostructures as platforms for metal enhanced fluorescence of the intrinsic emission of biomolecules in the ultra-violet

Mustafa Chowdhury, Krishanu Ray, Stephen Gray, James Pond, Joseph Lakowicz

Proceedings Volume 7577, 75770O (2010) https://doi.org/10.1117/12.841449

KEYWORDS: Aluminum, Nanoparticles, Luminescence, Ultraviolet radiation, Metals, Finite-difference time-domain method, Nanostructures, Near field, Particles, Quartz

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Proceedings Article | 3 March 2010 Paper

Metal-enhanced intrinsic fluorescence of proteins and label-free bioassays

Krishanu Ray, Henryk Szmacinski, Mustafa Chowdhury, Joseph Lakowicz

Proceedings Volume 7577, 75770K (2010) https://doi.org/10.1117/12.840412

KEYWORDS: Proteins, Luminescence, Aluminum, Quartz, Metals, Nanostructuring, Nanostructures, Scanning electron microscopy, Ultraviolet radiation, Gold

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Proceedings Article | 25 February 2010 Paper

Metal nanoparticle fluorophore: a powerful fluorescence probe in single cell imaging

Jian Zhang, Yi Fu, Richard Zhao, Joseph Lakowicz

Proceedings Volume 7571, 75710N (2010) https://doi.org/10.1117/12.842550

KEYWORDS: Metals, Nanoparticles, Molecules, Luminescence, Receptors, Atmospheric particles, Confocal microscopy, Particles, Silver, Silica

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Proceedings Article | 11 February 2010 Paper

Plasmon-controlled fluorescence: applications to sensing and single molecule detection

Yi Fu, Jian Zhang, Joseph Lakowicz

Proceedings Volume 7569, 756903 (2010) https://doi.org/10.1117/12.850765

KEYWORDS: Molecules, Silver, Luminescence, Fluorescence resonance energy transfer, Metals, Proteins, Particles, Glasses, Signal to noise ratio, Nanostructures

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Proceedings Article | 25 February 2009 Paper

Fluorophore conjugated silver nanoparticles: a time-resolved fluorescence correlation spectroscopic study

Krishanu Ray, Jian Zhang, Joseph Lakowicz

Proceedings Volume 7185, 71850C (2009) https://doi.org/10.1117/12.808958

KEYWORDS: Silver, Particles, Luminescence, Fluorescence correlation spectroscopy, Fluorescence spectroscopy, Molecules, Metals, Spectroscopy, Nanoparticles, Time resolved spectroscopy

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Proceedings Article | 25 February 2009 Paper

Plasmonic-controlled single molecule fluorescence near defined metallic nanostructures

Yi Fu, Jian Zhang, Joseph Lakowicz

Proceedings Volume 7185, 71850U (2009) https://doi.org/10.1117/12.811907

KEYWORDS: Metals, Molecules, Luminescence, Silver, Particles, Nanoparticles, Nanostructures, Absorption, Plasmons, Nanocomposites

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Proceedings Article | 18 February 2009 Paper

Computational study of the interaction of fluorophores with various metallic nanoparticle systems

Mustafa Chowdhury, Stephen Gray, James Pond, Joseph Lakowicz

Proceedings Volume 7192, 71920F (2009) https://doi.org/10.1117/12.809181

KEYWORDS: Nanoparticles, Silver, Metals, Particles, Luminescence, Near field, Computing systems, Finite-difference time-domain method, Atmospheric particles, Systems modeling

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Proceedings Article | 21 February 2008 Paper

Measurement and monitoring of the production of proteins during cell culture using plasmonic structures

Henryk Szmacinski, Derek Smith, Michael Hanson, Govind Rao, Joseph Lakowicz

Proceedings Volume 6869, 68690Z (2008) https://doi.org/10.1117/12.778490

KEYWORDS: Modulation, Luminescence, Phase shifts, Silver, Phase shift keying, Phase measurement, Statistical analysis, Calibration, Glasses, Proteins

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Proceedings Article | 21 February 2008 Paper

Use of aluminum films as substrates for enhanced fluorescence in the ultraviolet-blue spectral region

Mustafa Chowdhury, Krishanu Ray, Joseph Lakowicz

Proceedings Volume 6869, 68690E (2008) https://doi.org/10.1117/12.760724

KEYWORDS: Aluminum, Luminescence, Quartz, Metals, Finite-difference time-domain method, Near field, Particles, Nanoparticles, Scanning electron microscopy, Plasmons

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Proceedings Article | 21 February 2008 Paper

Single-molecule spectroscopy of semiconductor nanocrystals on plasmonic nanostructures

Krishanu Ray, Ramachandram Badugu, Joseph Lakowicz

Proceedings Volume 6869, 68690A (2008) https://doi.org/10.1117/12.760750

KEYWORDS: Nanocrystals, Luminescence, Glasses, Molecules, Silver, Single molecule spectroscopy, Plasmonics, Nanostructures, Fluorescence spectroscopy, Metals

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Proceedings Article | 21 February 2008 Paper

Single molecule photophysics near metallic nanostructures

Jian Zhang, Yi Fu, Krishanu Ray, Mustafa Chowdhury, Henryk Szmacinski, Kazimierz Nowaczyk, Joseph Lakowicz

Proceedings Volume 6862, 68620S (2008) https://doi.org/10.1117/12.770381

KEYWORDS: Metals, Particles, Silver, Luminescence, Molecules, Atmospheric particles, Finite-difference time-domain method, Nanoparticles, Fluorescence resonance energy transfer, Plasmons

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Proceedings Article | 27 February 2006 Paper

Ultrasensitive detection in optically dense physiological media: applications to fast reliable biological assays

Evgenia Matveeva, Ignacy Gryczynski, Klaus Berndt, Joseph Lakowicz, Ewa Goldys, Zygmunt Gryczynski

Proceedings Volume 6092, 60920K (2006) https://doi.org/10.1117/12.648112

KEYWORDS: Blood, Signal detection, Luminescence, Glasses, Silver, Surface plasmons, Metals, Statistical analysis, Biomedical optics, Fluorescence spectroscopy

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Proceedings Article | 23 February 2006 Open Access

Plasmon-controlled fluorescence: a new detection technology

Joseph Lakowicz, Mustafa Chowdhury, Krishanu Ray, Jian Zhang, Yi Fu, Ramachandram Badugu, Chandran Sabanayagam, Kazimierz Nowaczyk, Henryk Szmacinski, Kadir Aslan, Chris Geddes

Proceedings Volume 6099, 609909 (2006) https://doi.org/10.1117/12.673106

KEYWORDS: Plasmons, Luminescence, Metals, Particles, Plasmonics, Silver, Surface plasmons, Absorption, Near field, Scattering

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SPIE Journal Paper | 1 May 2005 Open Access

Application of microscopic Förster resonance energy transfer to cytological diagnosis of the thyroid tumors

Shin-ichi Murata, Petr Herman, Masanori Iwashina, Kunio Mochizuki, Tadao Nakazawa, Tetsuo Kondo, Nobuki Nakamura, Joseph Lakowicz, Ryohei Katoh

JBO, Vol. 10, Issue 03, 034008, (May 2005) https://doi.org/10.1117/12.10.1117/1.1924667

KEYWORDS: Fluorescence resonance energy transfer, Tumors, Luminescence, Image filtering, Electronic filtering, Distance measurement, Resonance energy transfer, Image segmentation, Medicine, Molecules

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Proceedings Article | 1 April 2005 Paper

Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

Evgenia Matveeva, Joanna Malicka, Ignacy Gryczynski, Joseph Lakowicz, Zygmunt Gryczynski

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.593430

KEYWORDS: Metals, Glasses, Silver, Prisms, Surface plasmons, Reflectivity, Thin films, Luminescence, Refractive index, Plasmons

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Proceedings Article | 1 April 2005 Paper

Multi-analyte multicolor enhanced fluorescent immunoassay on silver island-coated surface

Evgenia Matveeva, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.591685

KEYWORDS: Glasses, Luminescence, Optical filters, Silver, Signal detection, Electronic filtering, Reflection, Coating, Proteins, Statistical analysis

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Proceedings Article | 31 March 2005 Paper

Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska, Evgenia Matveeva, Ignacy Gryczynski, Robert Flower, Joanna Malicka, Joseph Lakowicz, Slawomir Makowiec, Zygmunt Gryczynski

Proceedings Volume 5703, (2005) https://doi.org/10.1117/12.593426

KEYWORDS: Luminescence, Silver, Nanoparticles, Proteins, Glasses, Magnesium, Particles, Atmospheric particles, Cameras, Optical imaging

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Proceedings Article | 1 July 2004 Paper

Metal-enhanced fluorescence: a novel approach to ultra-sensitive fluorescence sensing assay platforms

Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Evgenia Matveeva, Chris Geddes, Kadir Aslan, Joseph Lakowicz

Proceedings Volume 5321, (2004) https://doi.org/10.1117/12.530072

KEYWORDS: Luminescence, Metals, Particles, Silver, Quantum efficiency, Absorption, Sensors, Refractive index, Fluorescence spectroscopy, Atmospheric particles

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Proceedings Article | 1 July 2004 Paper

Enhanced chemical fluorescence-based sensing using metallic nano-composites

Henryk Szmacinski, Vincent Pugh, Wayne Moore, Chris Geddes, Kadir Aslan, Joseph Lakowicz

Proceedings Volume 5318, (2004) https://doi.org/10.1117/12.536415

KEYWORDS: Silver, Calcium, Nanoparticles, Luminescence, Quantum efficiency, Glasses, Absorption, Biological and chemical sensing, Silica, Silicon

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Proceedings Article | 1 July 2004 Paper

Complexation of dextran and glucose with thiolate boronic acid capped on nanoparticle

Jian Zhang, Chris Geddes, Joseph Lakowicz

Proceedings Volume 5321, (2004) https://doi.org/10.1117/12.530059

KEYWORDS: Particles, Luminescence, Glucose, Absorbance, Silver, Nanoparticles, Plasmons, Transmission electron microscopy, Spectroscopy, Atmospheric particles

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Proceedings Article | 1 July 2004 Paper

DNA hybridization using surface plasmon-coupled emission

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 5321, (2004) https://doi.org/10.1117/12.530001

KEYWORDS: Metals, Surface plasmons, Silver, Glasses, Prisms, Proteins, Luminescence, Reflectivity, Quantum efficiency, Quartz

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Proceedings Article | 21 June 2004 Paper

Long-wavelength long-lifetime luminophores for cellular and tissue imaging

Zygmunt Gryczynski, Badri Maliwal, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.533913

KEYWORDS: Quantum efficiency, Luminescence, Energy transfer, Energy efficiency, Resolution enhancement technologies, Absorption, Tissues, Fluorescence spectroscopy, Microscopy, Resonance energy transfer

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Proceedings Article | 16 June 2004 Paper

Plasmonic tunable glucose sensing based on the dissociation of Con A-aggregated dextran-coated gold colloids

Chris Geddes, Kadir Aslan, Jian Zhang, Joseph Lakowicz

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.530156

KEYWORDS: Gold, Nanoparticles, Glucose, Absorbance, Absorption, Plasmonics, Plasmons, Transmission electron microscopy, Molecular aggregates, Nanostructures

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Proceedings Article | 16 June 2004 Paper

Surface plasmon-coupled directional fluorescence emission

Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski, Kazimierz Nowaczyk, Joseph Lakowicz

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.530048

KEYWORDS: Prisms, Surface plasmons, Metals, Silver, Luminescence, Refractive index, Glasses, Reflectivity, Absorption, Absorbance

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Proceedings Article | 16 June 2004 Paper

Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.530091

KEYWORDS: Glasses, Luminescence, Silver, Signal detection, Absorbance, Particles, Coating, Fluorescent markers, Heart, Medicine

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Proceedings Article | 16 June 2004 Paper

Advances in surface-enhanced fluorescence

Joseph Lakowicz, Chris Geddes, Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski, Kadir Aslan, Joanna Lukomska, Jun Huang

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.542458

KEYWORDS: Silver, Luminescence, Quartz, Particles, Absorption, Metals, Atmospheric particles, Glasses, Quantum efficiency, Nanorods

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Proceedings Article | 14 June 2004 Paper

Luminescent blinking from silver, gold, and copper nanostructures: a new class of probes for imaging and localization

Chris Geddes, Ignacy Gryczynski, Alex Parfenov, Kadir Aslan, Joseph Lakowicz

Proceedings Volume 5329, (2004) https://doi.org/10.1117/12.530063

KEYWORDS: Silver, Glasses, Gold, Luminescence, Copper, Nanostructures, Absorption, Fractal analysis, Microscopes, Electrodes

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Proceedings Article | 10 June 2004 Paper

A glucose-sensing contact lens: a new approach to noninvasive continuous physiological glucose monitoring

Ramachandram Badugu, Joseph Lakowicz, Chris Geddes

Proceedings Volume 5317, (2004) https://doi.org/10.1117/12.530060

KEYWORDS: Glucose, Luminescence, Sensors, Blood, Positron emission tomography, Contact lenses, System on a chip, Polymers, Nitrogen, Americium

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Proceedings Article | 12 September 2003 Paper

Dual-emitting biosensors for glucose and glutamine from genertically engineered E. coli binding proteins

Leah Tolosa, Xudong Ge, Yordan Kostov, Joseph Lakowicz, Govind Rao

Proceedings Volume 4967, (2003) https://doi.org/10.1117/12.485718

KEYWORDS: Glucose, Proteins, Ruthenium, Sensors, Yeast, Modulation, Luminescence, Biosensors, Biochemistry, Biological research

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SPIE Journal Paper | 1 July 2003 Open Access

Metal-enhanced emission from indocyanine green: a new approach to in vivo imaging

Joanna Malicka, Ignacy Gryczynski, Chris Geddes, Joseph Lakowicz

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1578643

KEYWORDS: Silver, Quartz, Metals, Luminescence, Particles, Indocyanine green, Quantum efficiency, Molecules, Atmospheric particles, Absorption

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SPIE Journal Paper | 1 October 2002 Open Access

Charge transfer fluorescent probes using boronic acids for monosaccharide signaling

Nicholas DiCesare, Joseph Lakowicz

JBO, Vol. 7, Issue 04, (October 2002) https://doi.org/10.1117/12.10.1117/1.1502263

KEYWORDS: Glucose, Luminescence, Electrons, Boron, Fluorescence spectroscopy, Spectroscopy, Blood, Biological research, Confocal microscopy, Analytical research

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Proceedings Article | 21 June 2002 Paper

Biomedical applications of radiative decay engineering

Joseph Lakowicz, Ignacy Gryczynski, Joanna Malicka, Yibing Shen, Zygmunt Gryczynski

Proceedings Volume 4626, (2002) https://doi.org/10.1117/12.472114

KEYWORDS: Silver, Particles, Metals, Quantum efficiency, Rhodamine B, Luminescence, Absorption, Resonance energy transfer, Molecules, Biomedical optics

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Proceedings Article | 4 June 2002 Paper

Wavelength ratiometric fluorescent probes for glucose

Nicholas DiCesare, Joseph Lakowicz

Proceedings Volume 4625, (2002) https://doi.org/10.1117/12.469790

KEYWORDS: Glucose, Luminescence, Electrons, Blood, Boron, Tissue optics, Fluorescence spectroscopy, Temperature metrology, Proteins, Sensing systems

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SPIE Journal Paper | 1 July 2001 Open Access

Fluorescence properties of AB633 and AB670 in plasma and whole blood

Omoefe Abugo, Petr Herman, Joseph Lakowicz

JBO, Vol. 6, Issue 03, (July 2001) https://doi.org/10.1117/12.10.1117/1.1381053

KEYWORDS: Blood, Plasma, Anisotropy, Luminescence, Data modeling, Macromolecules, Absorption, Rhodamine, Argon ion lasers, Phase shift keying

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Proceedings Article | 10 May 2001 Paper

Glucose-sensing proteins from mesophilic and thermophilic bacteria as new tools in diabetes monitoring

S. D'Auria, Mose Rossi, Joseph Lakowicz

Proceedings Volume 4252, (2001) https://doi.org/10.1117/12.426739

KEYWORDS: Glucose, Proteins, Luminescence, Sensors, Polarization, Polarizers, Bacteria, Blood, Fluorescence spectroscopy, Microorganisms

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SPIE Journal Paper | 1 September 2000

Simple apparatus for polarization sensing of analytes

Zygmunt Gryczynski, Ignacy Gryczynski, Joseph Lakowicz

OE, Vol. 39, Issue 09, (September 2000) https://doi.org/10.1117/12.10.1117/1.1286521

KEYWORDS: Polarizers, Polarization, Glucose, Sensors, Luminescence, Statistical analysis, Proteins, Optical engineering, Ultraviolet radiation

Proceedings Article | 11 April 2000 Paper

Fluorescent measurements in whole blood and plasma using red-emitting dyes

Omoefe Abugo, Petr Herman, Joseph Lakowicz

Proceedings Volume 3913, (2000) https://doi.org/10.1117/12.382036

KEYWORDS: Blood, Plasma, Absorption, Anisotropy, Luminescence, Modulation, Rhodamine, Facial recognition systems, Data modeling, Scattering

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Proceedings Article | 18 November 1999 Paper

Fluorescence polarization sensing

Zygmunt Gryczynski, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 3857, (1999) https://doi.org/10.1117/12.370274

KEYWORDS: Polarization, Sensors, Luminescence, Polarizers, Oxygen, Calcium, Visualization, Statistical analysis, Measurement devices, Lamps

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SPIE Journal Paper | 1 October 1999 Open Access

Modulation sensing of fluorophores in tissue: a new approach to drug compliance monitoring

Omoefe Abugo, Zygmunt Gryczynski, Joseph Lakowicz

JBO, Vol. 4, Issue 04, (October 1999) https://doi.org/10.1117/12.10.1117/1.429956

KEYWORDS: Modulation, Tissues, Ruthenium, Skin, Phase shift keying, Rhodamine, Indocyanine green, Absorption, Scattering, Tissue optics

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SPIE Journal Paper | 1 October 1999 Open Access

Polarization-based sensing of glucose using an oriented reference film

Joseph Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Lisa Randers-Eichhorn, Govind Rao

JBO, Vol. 4, Issue 04, (October 1999) https://doi.org/10.1117/12.10.1117/1.429955

KEYWORDS: Polarization, Glucose, Proteins, Sensors, Anisotropy, Luminescence, Polarizers, Holographic optical elements, Light sources, Biological and chemical sensing

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Proceedings Article | 2 July 1999 Paper

Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium

Adrian Hamburger, Zygmunt Gryczynski, Joseph Lakowicz, Keith Sommers

Proceedings Volume 3600, (1999) https://doi.org/10.1117/12.351040

KEYWORDS: Luminescence, Proteins, Heart, Tissues, Injuries, Dye lasers, Ischemia, Fluorescence spectroscopy, Transplantation, Surgery

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Proceedings Article | 3 May 1999 Paper

Preliminary studies on the use of modulation sensing for noninvasive monitoring of drug compliance

Omoefe Abugo, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347548

KEYWORDS: Modulation, Tissues, Ruthenium, Skin, Luminescence, Phase shift keying, Rhodamine, Rhodium, Light scattering, Data modeling

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Proceedings Article | 3 May 1999 Paper

Lifetime-based sensing of glucose using luminescent ruthenium (II) metal complex

Zakir Murtaza, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347552

KEYWORDS: Glucose, Ruthenium, Metals

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Proceedings Article | 3 May 1999 Paper

DNA dynamics by using highly luminescent phosphine complex of osmium (II)

Zakir Murtaza, Henryk Malak, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347550

KEYWORDS: Metals, Osmium, Switches, Oxygen, Anisotropy, Fluorescence anisotropy, Ruthenium, Argon, Quantum efficiency, Time metrology

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Proceedings Article | 3 May 1999 Paper

Fluorescence sensing of glucose

Leah Tolosa, Ignacy Gryczynski, Lisa Randers-Eichhorn, Jonathan Dattelbaum, Govind Rao, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347544

KEYWORDS: Glucose, Modulation, Proteins, Sensors, Luminescence, Ruthenium, Phase shift keying, Blood, Lithium, Phase measurement

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Proceedings Article | 3 May 1999 Paper

Long-lifetime and long-wavelength osmium (II) metal complexes containing polypyridine ligands: excellent red fluorescent dyes for biophysics and for sensors

Zakir Murtaza, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347549

KEYWORDS: Osmium, Anisotropy, Oxygen, Sensors, Metals, Biophysics, Luminescence, Polarization, Macromolecules, Bioalcohols

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Proceedings Article | 3 May 1999 Paper

Novel methods for fluorescence sensing

Joseph Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan Dattelbaum, Leah Tolosa, Govind Rao

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347525

KEYWORDS: Polarization, Sensors, Oxygen, Anisotropy, Polarizers, Glucose, Luminescence, Silicon, Chemical analysis, Biological and chemical sensing

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SPIE Journal Paper | 1 April 1999 Open Access

A Letter from the Editor

Joseph Lakowicz

JBO, Vol. 4, Issue 02, (April 1999) https://doi.org/10.1117/12.10.1117/1.429908

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Proceedings Article | 1 May 1998 Paper

Fluorescence anisotropy controlled by light quenching

Ignacy Gryczynski, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307076

KEYWORDS: Quenching (fluorescence), Anisotropy, Fluorescence anisotropy, Polarization, Luminescence, Molecules, Dye lasers, Absorption, Polarizers, Proteins

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Proceedings Article | 1 May 1998 Paper

Long-lived highly luminescent rhenium (I) metal-ligand complex as a probe of biomolecules

Felix Castellano, Xiang-Qun Guo, Li Li, Henryk Szmacinski, Jeffrey Sipior, Joseph Lakowicz

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307064

KEYWORDS: Anisotropy, Luminescence, Proteins, Quantum efficiency, Oxygen, Polarization, Rhenium, Argon, Absorption, Magnesium

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Proceedings Article | 1 May 1998 Paper

Multiphoton excitation of fluorescence

Joseph Lakowicz, Ignacy Gryczynski

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307075

KEYWORDS: Anisotropy, Luminescence, Proteins, Absorption, Spectroscopy, Fluorescence spectroscopy, Fluorescence anisotropy, Photons, Sapphire lasers, Microscopy

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Proceedings Article | 10 April 1998 Paper

Fluorescence detection of camptothecin anticancer drugs by two-photon excitation

Thomas Burke, Magda Malak, David Bom, Dennis Curran, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 3259, (1998) https://doi.org/10.1117/12.307334

KEYWORDS: Blood, Fluorescence spectroscopy, Plasma, Luminescence, Cancer, Absorption, Tissues, Polarization, Chemistry, Oncology

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Proceedings Article | 7 May 1997 Paper

Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation

Thomas Burke, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273532

KEYWORDS: Plasma, Blood, Luminescence, Fluorescence spectroscopy, Cancer, Oncology, Tissues, Skin, Translucency, Near infrared

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Proceedings Article | 7 May 1997 Paper

Two-color two-photon excitation of fluorescence

Joseph Lakowicz, Ignacy Gryczynski, Henryk Malak, Zygmunt Gryczynski

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273539

KEYWORDS: Luminescence, Anisotropy, Absorption, Picosecond phenomena, Signal processing, Polarization, Fluorescence anisotropy, Microscopy, Proteins, Dye lasers

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SPIE Journal Paper | 2 January 1997 Open Access

Wavelength-selective light quenching of biochemical fluorophores

Ignacy Gryczynski, Jozef Kusba, Joseph Lakowicz

JBO, Vol. 2, Issue 01, (January 1997) https://doi.org/10.1117/12.10.1117/12.259609

KEYWORDS: Quenching (fluorescence), Proteins, Macromolecules, Luminescence, Phase shift keying, Dye lasers, Solids, Modulation, Diffusion, Picosecond phenomena

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SPIE Journal Paper | 1 October 1996 Open Access

Three-photon excitation of 2,5-bis(4-biphenyl)oxazole: steady-state and time-resolved intensities and anisotropies

Ignacy Gryczynski, Henryk Malak, Stefan Hell, Joseph Lakowicz

JBO, Vol. 1, Issue 04, (October 1996) https://doi.org/10.1117/12.10.1117/12.250670

KEYWORDS: Anisotropy, Sapphire lasers, Fluorescence anisotropy, Photons, Modulation, Femtosecond phenomena, Luminescence, Absorption, Signal attenuation, Fluorescence spectroscopy

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Proceedings Article | 7 May 1996 Paper

Fluorescence-lifetime-based sensors: oxygen sensing and other biomedical applications

Lisa Randers-Eichhorn, Roscoe Bartlett, Jeffrey Sipior, Douglas Frey, Gary Carter, Joseph Lakowicz, Govind Rao

Proceedings Volume 2681, (1996) https://doi.org/10.1117/12.239568

KEYWORDS: Oxygen, Liquids, Sensors, Diffusion, Resistance, Tissues, Silicon, Glucose, Fiber optics sensors, Optical sensors

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SPIE Journal Paper | 1 April 1996 Open Access

Editorial: A Letter from the Editor

Joseph Lakowicz

JBO, Vol. 1, Issue 02, (April 1996) https://doi.org/10.1117/12.10.1117/12.240220

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SPIE Journal Paper | 2 January 1996 Open Access

Directly modulated diode laser frequency doubled in a KTP waveguide as an excitation source for CO₂ and O2 phase fluorometric sensors

Raja Holavanahali, Romauld M., Gary Carter, Govind Rao, Jeffrey Sipior, Joseph Lakowicz, John Bierlein

JBO, Vol. 1, Issue 01, (January 1996) https://doi.org/10.1117/12.10.1117/12.227105

KEYWORDS: Sensors, Modulation, Semiconductor lasers, Waveguides, Phase shift keying, Ferroelectric materials, Luminescence, Oxygen, Carbon monoxide, Fiber optics sensors

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SPIE Journal Paper | 2 January 1996 Open Access

Three-photon excitation in fluorescence microscopy

Stefan Hell, Karsten Bahlmann, Martin Schrader, Aleksi Soini, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

JBO, Vol. 1, Issue 01, (January 1996) https://doi.org/10.1117/12.10.1117/12.229062

KEYWORDS: Luminescence, Microscopes, Microscopy, Absorption, Rhodamine, Photons, Sapphire lasers, Mode locking, Absorbance, Objectives

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SPIE Journal Paper | 1 January 1996 Open Access

Editorial: A Letter from the Editor

Joseph Lakowicz

JBO, Vol. 1, Issue 01, (January 1996) https://doi.org/10.1117/12.10.1117/12.230917

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Proceedings Article | 8 May 1995 Paper

Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics and fluorescence polarization immunoassay

Joseph Lakowicz, Ewald Terpetschnig, Henryk Szmacinski, Henryk Malak

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208497

KEYWORDS: Polarization, Luminescence, Proteins, Anisotropy, Fluorescence anisotropy, Ruthenium, Diffusion, Absorption, Motion measurement, Picosecond phenomena

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Proceedings Article | 8 May 1995 Paper

Lifetime-based sensing in highly scattering media

Henryk Szmacinski, Joseph Lakowicz

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208549

KEYWORDS: Phase shift keying, Light scattering, Modulation, Sensors, Luminescence, Scattering media, Phase measurement, Skin, Scattering, Chemistry

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Proceedings Article | 17 August 1994 Paper

End-to-end diffusion coefficients and distance distributions from fluorescence energy transfer measurements: enhanced resolution by using multiple donors with different lifetimes

Ignacy Gryczynski, Joseph Lakowicz, Jozef Kusba

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182763

KEYWORDS: Diffusion, Energy transfer, Luminescence, Molecules, Resolution enhancement technologies, Molecular energy transfer, Proteins, Solids, Distance measurement, Fluorescence resonance energy transfer

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Proceedings Article | 17 August 1994 Paper

Control of excited-state population by light quenching of fluorescence

Ignacy Gryczynski, Jozef Kusba, Valery Bogdanov, Joseph Lakowicz

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182784

KEYWORDS: Quenching (fluorescence), Anisotropy, Rhodamine B, Polarization, Luminescence, Absorption, Picosecond phenomena, Fluorescence anisotropy, Dye lasers, Pulsed laser operation

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Proceedings Article | 17 August 1994 Paper

Center for Fluorescence Spectroscopy: advanced studies of fluorescence dynamics, lifetime imaging, clinical sensing, two-photon excitation, and light quenching

Joseph Lakowicz, Henryk Malak, Ignacy Gryczynski, Henryk Szmacinski, Jozef Kusba, Engin Akkaya, Ewald Terpetschnig, Michael Johnson

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182725

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Proteins, Fluorescence spectroscopy, Time resolved spectroscopy, Quenching (fluorescence), Ultraviolet radiation, Near infrared, Picosecond phenomena, Fluorescence anisotropy

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Proceedings Article | 17 August 1994 Paper

Distance-dependent fluorescence quenching of N-acetyl-L-tryptophanamide by acrylamide and iodide

Bogumil Zelent, Ignacy Gryczynski, Jozef Kusba, Michael Johnson, Joseph Lakowicz

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182750

KEYWORDS: Luminescence, Data modeling, Molecules, Quantum efficiency, Solids, Modulation, Diffusion, Chemical species, Proteins, Potassium

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Proceedings Article | 17 August 1994 Paper

One- and two-pulse theory of light quenching

Jozef Kusba, Valery Bogdanov, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182737

KEYWORDS: Quenching (fluorescence), Anisotropy, Polarization, Fluorescence anisotropy, Luminescence, Molecules, Modulation, Absorption, Diffusion, Solids

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Proceedings Article | 17 August 1994 Paper

Synthesis of squaraine-N-hydroxysuccinimide esters and their biological application as long-wavelength fluorescent labels

Ewald Terpetschnig, Henryk Szmacinski, Joseph Lakowicz

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182771

KEYWORDS: Proteins, Quantum efficiency, Luminescence, Semiconductor lasers, Fluorescent markers, Water, Absorption, Phase shift keying, Modulation, Fluorescence spectroscopy

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Proceedings Article | 17 August 1994 Paper

Fluorescence intensity and anisotropy decays of the DNA stain Hoechst 33342 resulting from one-photon and two-photon excitation

Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182738

KEYWORDS: Anisotropy, Fluorescence anisotropy, Bioalcohols, Holographic optical elements, Absorption, Luminescence, Dye lasers, Molecules, Photons, Microscopy

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Proceedings Article | 21 July 1994 Paper

Emerging biomedical applications of time-resolved fluorescence spectroscopy

Joseph Lakowicz, Henryk Szmacinski, Peter Koen

Proceedings Volume 2136, (1994) https://doi.org/10.1117/12.180786

KEYWORDS: Blood, Glucose, Luminescence, Semiconductor lasers, Flow cytometry, Fluorescence lifetime imaging, Time resolved spectroscopy, Calcium, Fluorescence resonance energy transfer, Sensors

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Proceedings Article | 21 July 1994 Paper

Lifetime-based optical sensing of pH using resonance energy transfer in sol-gel sensors

Jeffrey Sipior, Shabbir Bambot, Joseph Lakowicz, Govind Rao

Proceedings Volume 2136, (1994) https://doi.org/10.1117/12.180800

KEYWORDS: Sensors, Sol-gels, Luminescence, Fluorescence resonance energy transfer, Phase measurement, Modulation, Coating, Glasses, Absorption, Semiconductor lasers

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Proceedings Article | 19 May 1994 Paper

Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy

Christina Burch, Joseph Lakowicz, Eva Sevick-Muraca

Proceedings Volume 2135, (1994) https://doi.org/10.1117/12.176003

KEYWORDS: Absorption, Scattering, Tissues, Light scattering, Modulation, Phase shift keying, Light, Diffusion, Spectroscopy, Luminescence

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Proceedings Article | 14 September 1993 Paper

Time-dependent photon migration imaging in two dimensions: a method for detection and localization of absorbers in tissuelike media

Eva Sevick-Muraca, Joan Frisoli, Christina Hutchinson, Henryk Szmacinski, Kazimierz Nowaczyk, Michael Johnson, Joseph Lakowicz

Proceedings Volume 1888, (1993) https://doi.org/10.1117/12.154662

KEYWORDS: Modulation, Adaptive optics, Sensors, Monte Carlo methods, Phase modulation, Photon transport, Tissue optics, Biomedical optics, Phase shift keying, Scattering

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Proceedings Article | 24 June 1993 Paper

Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging

Joseph Lakowicz, Henryk Szmacinski, Richard Thompson

Proceedings Volume 1895, (1993) https://doi.org/10.1117/12.146718

KEYWORDS: Modulation, Phase shift keying, Luminescence, Calcium, Glucose, Chemistry, Diagnostics, Energy transfer, Fluorescence lifetime imaging, Blood

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Proceedings Article | 18 May 1993 Paper

Homogeneous model immunoassay of thyroxine by phase-modulation fluorescense spectroscopy

Alvydas Ozinskas, Henryk Malak, Judy Britz, Richard Thompson, Peter Koen, Joseph Lakowicz

Proceedings Volume 1885, (1993) https://doi.org/10.1117/12.144697

KEYWORDS: Luminescence, Energy transfer, Phase shift keying, Modulation, Phase measurement, Sodium, Fluorescence resonance energy transfer, Blood, Polarization, Absorption

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Proceedings Article | 1 January 1993 Paper

Subnanosecond time-resolved imaging using a rf phase-sensitive image converter camera

Klaus Berndt, Joseph Lakowicz

Proceedings Volume 1801, (1993) https://doi.org/10.1117/12.145795

KEYWORDS: Modulation, Image intensifiers, Phase shift keying, Cameras, Image processing, High speed photography, Photonics, Sensors, Light sources, Mode locking

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Proceedings Article | 16 April 1992 Paper

Fluorescence-lifetime-based sensing of blood gases and cations

Joseph Lakowicz, Henryk Szmacinski, Klaus Berndt

Proceedings Volume 1648, (1992) https://doi.org/10.1117/12.58296

KEYWORDS: Sensors, Modulation, Phase shift keying, Luminescence, Phase measurement, Phase modulation, Fiber optics, Fiber optics sensors, Modulators, Helium neon lasers

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Proceedings Article | 1 April 1992 Paper

Site-to-site diffusion in proteins as observed by energy transfer and frequency-domain fluorometry

Joseph Lakowicz, Ignacy Gryczynski, Jozef Kusba, Wieslaw Wiczk, Henryk Szmacinski, Michael Johnson

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58215

KEYWORDS: Diffusion, Energy transfer, Proteins, Laser spectroscopy, Biochemistry, Molecules, Modulation, Luminescence, Phase shift keying, Chemical oxygen iodine lasers

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Proceedings Article | 1 April 1992 Paper

Dimensionality of energy transfer between intercalated and surface-bound donor-acceptor pairs in DNA

Badri Maliwal, Jozef Kusba, Joseph Lakowicz

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58253

KEYWORDS: Energy transfer, Field effect transistors, Laser spectroscopy, Biochemistry, Adaptive optics, Luminescence, Phase shift keying, Modulation, Transition metals, Ions

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Proceedings Article | 1 April 1992 Paper

Energy transfer distance distributions recovered from a zinc finger peptide using time-resolved frequency-domain fluorometry

Peggy Eis, Joseph Lakowicz

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58246

KEYWORDS: Zinc, Energy transfer, Ions, Metals, Laser spectroscopy, Biochemistry, Molecules, Proteins, Luminescence, Quantum efficiency

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Proceedings Article | 1 April 1992 Paper

Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs

Ignacy Gryczynski, Zygmunt Gryczynski, Wieslaw Wiczk, Jozef Kusba, Joseph Lakowicz

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58257

KEYWORDS: Energy transfer, Distance measurement, Laser spectroscopy, Biochemistry, Macromolecules, Data modeling, Fluorescence resonance energy transfer, Modulation, Proteins, Molecules

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Proceedings Article | 1 April 1992 Paper

Fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission of hemoglobin measured with a 10-GHz fluorometer using front-face geometry on free liquid surface

Enrico Bucci, Zygmunt Gryczynski, Clara Fronticelli, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58249

KEYWORDS: Fluorescence anisotropy, Luminescence, Anisotropy, Picosecond phenomena, Liquids, Fluorometers, Solids, Laser spectroscopy, Biochemistry, Modulation

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Proceedings Article | 1 April 1992 Paper

Detection of a distance-dependent rate of quenching by frequency-domain fluorometry

Jozef Kusba, Ignacy Gryczynski, Henryk Szmacinski, Michael Johnson, Joseph Lakowicz

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58200

KEYWORDS: Data modeling, Diffusion, Luminescence, Laser spectroscopy, Biochemistry, Modulation, Phase shift keying, Biological research, Carbon, Optical simulations

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Proceedings Article | 1 April 1992 Paper

Fluorescence lifetime imaging of Ca2+ using visible wavelength excitation and emission

Joseph Lakowicz, Henryk Szmacinski, Kazimierz Nowaczyk, Michael Johnson

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58231

KEYWORDS: Phase shift keying, Modulation, Fluorescence lifetime imaging, Calcium, Luminescence, Sensors, Image intensifiers, Biochemistry, Laser spectroscopy, Microscopy

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Proceedings Article | 1 April 1992 Paper

Frequency-domain fluorescence of mutant cytochrome b5

Alexey Ladokhin, Henryk Malak, Michael Johnson, Joseph Lakowicz, L. Wang, A. Steggles, Peter Holloway

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58250

KEYWORDS: Luminescence, Proteins, Biochemistry, Laser spectroscopy, Energy transfer, Chromophores, Shape analysis, Quenching (fluorescence), Fluorescence spectroscopy, Spectroscopy

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Proceedings Article | 1 February 1992 Paper

Advances in frequency-domain flourometry; gigahertz instrumentation; time-dependent photo migration and flourescence lifetime imaging

Joseph Lakowicz

Proceedings Volume 1599, (1992) https://doi.org/10.1117/12.2322288

KEYWORDS: Modulation, Phase shift keying, Fluorescence lifetime imaging, Picosecond phenomena, Chemistry, Medicine, Photons, Sensors, Physics, Scattering

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Proceedings Article | 1 July 1991 Paper

Frequency-domain fluorescence spectroscopy: instrumentation and applications to the biosciences

Joseph Lakowicz, Ignacy Gryczynski, Henryk Malak, Michael Johnson, Gabor Laczko, Wieslaw Wiczk, Henryk Szmacinski, Jozef Kusba

Proceedings Volume 1435, (1991) https://doi.org/10.1117/12.44239

KEYWORDS: Modulation, Picosecond phenomena, Diffusion, Fluorescence spectroscopy, Phase shift keying, Time metrology, Luminescence, Sensors, Microchannel plates, Light sources

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Proceedings Article | 1 May 1991 Paper

Detection and localization of absorbers in scattering media using frequency-domain principles

Klaus Berndt, Joseph Lakowicz

Proceedings Volume 1431, (1991) https://doi.org/10.1117/12.44186

KEYWORDS: Modulation, Reflectivity, Photons, Scattering, Image intensifiers, Imaging spectroscopy, Time resolved spectroscopy, Tissue optics, Tissues, Phase shift keying

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