Unspecified Kai Chen Profile

Kai Chen

at Xidian Univ

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 16 March 2023 Poster + Paper

Label-free cell imaging based on multi-wavelength lensless digital holography

Kai Chen, Duofang Chen, Huidi Guan, Feng Chen, Xueli Chen

Proceedings Volume 12391, 123910T (2023) https://doi.org/10.1117/12.2655815

KEYWORDS: 3D image reconstruction, Holograms, Digital holography, Image restoration, Numerical simulations, Imaging systems, Phase retrieval, Compressed sensing, Holography, Image quality

Read Abstract +

Without any staining or labeling process, label-free cell imaging has less damage to cells and is suitable for longitudinal observation of cell behaviors. The lensless digital holographic microscopy, which directly uses photoelectric sensor chip to collect holographic images, has the advantages of low cost, non-invasive, large field of view and high resolution, and has been widely used in label-free cell imaging. However, when the classical angular spectrum method is used to recover the complex optical field in lensless digital holography, the twin image shows up and has a serious impact on image quality. To improve the quality of the reconstruction, a multi-wavelength lensless digital holography method is used for cell imaging in this paper. During numerical reconstruction, autofocus is performed firstly, and then the object light field is restored by the multi-wavelength phase retrieval (MWPR) iteration method combined with the compressive sensing (CS) strategy. The experiment results show that the reconstruction method based on multi-wavelength illumination combined with compressive sensing can effectively eliminate the influence of twin image and improve the reconstruction quality. Compared with the reconstruction results of single wavelength hologram, our method can not only obtain highresolution labeled cell images with large field of view, but also obtain high-quality non-labeled cell video, which can be used as an effective technique for cell imaging and dynamic continuous observation in the future.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years