Lightsheet fluorescence microscopy (LSFM) has rapidly progressed in the past decade from an emerging technology into
an established methodology. This progress has largely been driven by its suitability to developmental biology, where it
is able to give excellent spatial-temporal resolution over relatively large fields of view with good contrast and low
phototoxicity. In many respects it is superseding confocal microscopy. However, it is no magic bullet and still struggles
to image deeply in more highly scattering samples. Many solutions to this challenge have been presented, including,
Airy and Bessel illumination, 2-photon operation and deconvolution techniques. In this work, we show a comparison
between a simple but effective Gaussian beam illumination and Bessel illumination for imaging in chicken embryos.
Whilst Bessel illumination is shown to be of benefit when a greater depth of field is required, it is not possible to see any
benefits for imaging into the highly scattering tissue of the chick embryo.
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