Brain tumor, especially glioblastoma multiforme (GBM), is one of the most malignant tumors, which not only demands
perplexing treatment approaches but also requires potent and effective treatment modality to deal with recurrence of the tumor. Photodynamic therapy (PDT) is a treatment which has been recommended as a third-level treatment. We are
trying to investigate possibility of the PDT as an efficient adjuvant therapeutic modality for the treatment of brain tumor. Inhibition of tumor progression with photosensitizer was verified, in vitro. With micellar nanoscale drug delivery system, localization of the tumor was identified, in vivo, which is able to be referred as photodynamic diagnosis. With consequent results, we are suggesting photodynamic diagnosis and therapy is able to be performed simultaneously with our nanoscale drug delivery system.
Astrocyte, the most abundant cell type in the central nervous system, has been one of major topics in neuroscience. Even
though many tools have been developed for the analysis of astrocyte function, there has been no adequate tool that can
modulates astrocyte network without pharmaceutical or genetic interventions. Here we found that ultrashort pulsed laser
stimulation can induce label-free activation of astrocytes as well as apoptotic-like cell death in a dose-dependent manner.
Upon irradiation with high intensity pulsed lasers, the irradiated cells with short exposure time showed very rapid
mitochondria fragmentation, membrane blebbing and cytoskeletal retraction. We applied this technique to investigate in
vivo function of astrocyte network in the CNS: in the aspect of neurovascular coupling and blood-brain barrier. We
propose that this noninvasive technique can be widely applied for in vivo study of complex cellular network.
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