Genetically encoded light-sensitive proteins can be used to manipulate and observe cellular functions. According to
different modes of action, these proteins are divided into actuators like the blue-light gated cation channel
Channelrhodopsin-2 (ChR2) and detectors like the calcium sensor GCaMP. In order to optogenetically control and study
the activity of rat primary cortical neurons, we established a transduction procedure using recombinant Adeno-associated
viruses (rAAVs) as gene-ferries. Thereby, we achieved high transduction rates of these neurons with ChR2. In ChR2
expressing neurons, action potentials could be repeatedly and precisely elicited with laser pulses and measured via patch
clamp recording.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.