Dr. Mario Marini Profile

Dr. Mario Marini

at Univ degli Studi di Milano-Bicocca

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Publications (5)

Proceedings Article | 13 March 2024 Presentation

Rapid fabrication of a bioimaging micro-chip using two-photon polymerization

Behjat Kariman, Alessandra Nardini, Marco Grassi, Mario Marini, Claudio Conci, Margaux Bouzin, Maddalena Collini, Manuela Raimondi, Giuseppe Chirico, Roberto Osellame, Giulio Cerullo, Rebeca Martínez Vázquez

Proceedings Volume PC12875, PC1287503 (2024) https://doi.org/10.1117/12.3002218

KEYWORDS: Two photon polymerization, Fabrication, Biological imaging, Microlens, In vivo imaging, Windows, Lenses, Confocal microscopy, Voxels, Ultraviolet radiation

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Proceedings Article | 12 March 2024 Presentation + Paper

Non-linear excitation microscopy of live cells by using an implantable microscope objective-on-a-chip

A. Nardini, R. Martínez Vázquez, C. Conci, M. Grassi, M. Marini, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. Kariman, M. Raimondi, G. Chirico

Proceedings Volume 12846, 1284607 (2024) https://doi.org/10.1117/12.3001604

KEYWORDS: Microlens, Objectives, Ultraviolet radiation, Two photon polymerization, Microscopes, Fabrication, Scanning electron microscopy, Biological imaging, Microfabrication, Fluorescence

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Proceedings Article | 11 August 2023 Presentation + Paper

Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples

A. Nardini, R. Martínez Vázquez, M. Marini, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. Kariman, M. Farsari, E. Kabouraki, G. Chirico, M. Raimondi

Proceedings Volume 12622, 1262206 (2023) https://doi.org/10.1117/12.2672894

KEYWORDS: Microlens, Two photon polymerization, Nonlinear optics, Fabrication, Biological samples, Polymers, Biological imaging, Polymerization, In vivo imaging, Microscopy

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Proceedings Article | 27 May 2022 Paper

A miniaturized chip for 3D optical imaging of tissue regeneration in vivo

Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Rebeca Martinez, Roberto Osellame, Mario Marini, Margaux Bouzin, Maddalena Collini, Laura D'Alfonso, Elmina Kabouraki, Maria Farsari, Anthi Ranella, Nikos Kehagias, Giuseppe Chirico, Manuela Raimondi

Proceedings Volume 12144, 121440D (2022) https://doi.org/10.1117/12.2629824

KEYWORDS: Tissues, Microscopy, In vivo imaging, Confocal microscopy, Two photon polymerization, Tissue optics, Spatial light modulators, Multiphoton fluorescence microscopy

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Proceedings Article | 20 June 2021 Presentation

Development and characterization of proteinaceous multiphoton-fabricated microstructures with photo-thermal functionality

Mario Marini, Amirbahador Zeynali, Margaux Bouzin, Laura Sironi, Laura D'Alfonso, Piersandro Pallavicini, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 11786, 117861U (2021) https://doi.org/10.1117/12.2593350

KEYWORDS: Nanoparticles, Microfluidics, 3D microstructuring, Tunable lasers, Thermography, Spatial resolution, Proteins, Printing, Neurons, Multiphoton lithography

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We exploit two-photon laser writing to fabricate 3D biocompatible proteinaceous microstructures (∼1 to 50 𝜇m in lateral size) with tunable elasticity and photo-thermal activity in the near-infrared. Structure printing relies on the photo-crosslinking of the protein bovine serum albumin (BSA, 50 mg/mL) initiated by the Rose Bengal dye (2 mM concentration), whereas photo-thermal functionality is achieved by the dispersion of non-spherically symmetric metallic nanoparticles into the ink. Aiming at a subsequent application of the fabricated microstructures as platforms for cell growth and stimulation, we carry out a thorough characterization of their mechanical and photo-thermal properties. Preliminary data obtained by AFM indentation have quantified the structures Young modulus in the broad 100-1000 kPa range depending on the BSA concentration. Stiffness is further characterized here by subjecting the fabricated microstructures to steady flow in a microfluidic device, and by quantifying their real-time bending by a conventional transmitted light microscope. In parallel, we focus on the optimization of the photo-thermal activity of the structures. Anisotropic gold nanoparticles, dispersed in the ink, get trapped into the structure during photo-crosslinking and lead to localized heat release upon excitation in the near-infrared. The temperature increment is rapidly (∼1 s) reached and maintained under continuous wave laser irradiation at 800 nm; the amplitude of the temperature variation is quantified as a function of the incident laser power by means of infrared thermography and is correlated to both the structure thickness and the nanoparticles concentration. The resulting spatially confined heat loads could be exploited to induce highly localized responses in cells. In this direction, proteinaceous photo-thermal microstructures can be used to physically induce the differentiation of cells (e.g. neurons or fibroblasts) in a spatially controlled manner.

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