Fluorescence microscopy based on non-linear optical phenomena has become a new perspective optical tool in biological imaging. This contribution is aimed at synthesis and micro/spectroscopic characterization of novel S,N-heteroarene-functionalized BODIPY-class dyes with potential application as fluorescence and NLO markers in high-resolution multiphoton microscopy. The molecules have been classified with respect to their NLO response and most perspective ones has been tested for potential applications on cultured cancer cells. Supported by: EU-H2020 871124 (Laserlab-Europe) and 810701 (LAMatCU), Slovak research grant agencies APVV-17-0324, APVV-21-0503, VEGA No. 1/0669/22 and VEGA No. 2/0070/21.
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