Plants have astonishing ability to yield and release a variety of organic and inorganic compounds into the rhizosphere known as root exudates. The normal yield of these root exudates can be enhanced by applying various abiotic stresses. In current research abiotic stresses i.e. physical and chemical have been used to get desired outcomes for root exudates of canola plant. Effect of abiotic stress on total phenolic content and antioxidant activity of root exudates has also been observed. The results showed that by applying abiotic stress the obtained yield came out in the range 1.56 ± 1.79 to 1.97 ± 1.74 percent/100 g DW which was 0.03 percent/100g DW without applying abiotic stress. In addition to this TPC results ranged 31.92 ± 2.22 - 511.3 ± 10.27 mg GAE/g of DW that was higher as compared to the value of control (11.2 ± 2.11 mg GAE/g of DW). The value of IC50 calculated by the DPPH radical scavenging assay was about 0.58 ± 0.02 - 1.71 ± 0.03 mg/mL with abiotic stress as compared to the value of control to be 2.1 ± 0.03 mg/mL that indicated the increasing antioxidant activity with respect to abiotic stress application.
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