The use of dynamic speckle laser, also called biospeckle laser (BSL), presents a series of challenges to its
adoption, as it is the case for its use in real time, and when ones desires to adjust the experimental setup. The
technical analysis of the BSL adopted in real time has reduced spatial resolution of the captured image, while the
adjustment of preparing an experiment still demands the trial of a specialist. In this work two approaches have been
proposed to improve the efficiency of BSL real-time adjustments to support the experimental setup. The use of the
method known as Motion History Image (MHI) was carry out over a series of BSL images. The MHI was the basis
for the implementation of a graphical interface for real-time identification of areas of activity, and then delimiting the
regions of interest. The results showed that the MHI was effective in the reproduction of the activities of speckle
patterns in real time without reducing image resolution, and as an instrument for delimit of regions of activity,
supporting the analyst in the choice of lighting adjustments and image assembling.
The biospeckle laser (BSL) has been applied in many areas of knowledge and a variety of approaches has been presented
to address the best results in biological and non-biological samples, in fast or slow activities, or else in defined flow of
materials or in random activities. The methodologies accounted in the literature consider the apparatus used in the image
assembling and the way the collected data is processed. The image processing steps presents in turn a variety of
procedures with first or second order statistics analysis, and as well with different sizes of data collected. One way to
access the biospeckle in defined flow, such as in capillary blood flow in alive animals, was the adoption of the image
contrast technique which uses only one image from the illuminated sample. That approach presents some problems
related to the resolution of the image, which is reduced during the image contrast processing. In order to help the
visualization of the low resolution image formed by the contrast technique, this work presents the three-dimensional
procedure as a reliable alternative to enhance the final image. The work based on a parallel processing, with the
generation of a virtual map of amplitudes, and maintaining the quasi-online characteristic of the contrast technique.
Therefore, it was possible to generate in the same display the observed material, the image contrast result and in
addiction the three-dimensional image with adjustable options of rotation. The platform also offers to the user the
possibility to access the 3D image offline.
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