KEYWORDS: Hyperspectral imaging, RGB color model, Elasticity, Education and training, Tissues, Image quality, Collagen, Digital imaging, Data modeling, Data conversion
SignificanceQuantification of elastic fiber in the tissue specimen is an important aspect of diagnosing different diseases. Though hematoxylin and eosin (H&E) staining is a routinely used and less expensive tissue staining technique, elastic and collagen fibers cannot be differentiated using it. So, in conventional pathology, special staining technique, such as Verhoeff’s van Gieson (EVG), is applied physically for this purpose. However, the procedure of EVG staining is very expensive and time-consuming.AimThe goal of our study is to propose a deep-learning-based computerized method for the generation of RGB EVG stained tissue from hyperspectral H&E stained one to save the time and cost of conventional EVG staining procedure.ApproachH&E stained hyperspectral image and EVG stained RGB whole slide image of human pancreatic tissue have been leveraged for this experiment. CycleGAN-based deep learning model has been proposed for digital stain conversion while images from source and target domains are of different modalities (hyperspectral and RGB) with different channel dimensions. A set of three basis functions have been introduced for calculating one of the losses of the proposed method, which retains the relevant features of EVG stained image within the reduced channel dimension of the H&E stained one.ResultsThe experimental results showed that a set of three basis functions including linear discriminant function and transmittance spectrum of eosin and hematoxylin better retained the essential properties of the elastic fiber to be discriminated from collagen fiber within the reduced dimension of the hyperspectral H&E stained image. Also, only a smaller number of paired training data with our proposed training method contributed significantly to the generation of more realistic EVG stained image with more precise identification of elastic fiber.ConclusionsRGB EVG stained image is generated from hyperspectral H&E stained image for which our model has performed two types of image conversion simultaneously: hyperspectral to RGB and H&E to EVG. The experimental results show that the intentionally designed set of three basis functions contains more relevant information and prove the effectiveness of our proposed method in generating realistic RGB EVG stained image from hyperspectral H&E stained one.
Quantifying elastic fiber in the tissue specimen is an important aspect of diagnosing different diseases. In conventional pathology, special staining technique such as EVG (Verhoeff’s Van Gieson) is applied physically for this purpose which is expensive and time-consuming procedure. Though H&E (Hematoxylin and Eosin) staining is routinely used, less expensive and most common tissue staining technique, elastic and collagen fibers cannot be differentiated using it. This study proposes a modified CycleGAN based unsupervised method for the computerized generation of RGB EVG stained tissue from hyperspectral H&E stained one to save the time and cost of conventional EVG staining procedure. Our proposed method is designed to utilize the sufficient spectral information provided by the H&E hyperspectral image (HSI) without reducing the spectral dimension. For doing so, we have faced challenges to calculate one of the training losses (identity loss) of CycleGAN that requires reducing the channel dimension of H&E HSI to be the same as RGB EVG stained image. We have addressed the issue by adopting intentionally designed three basis functions that can reduce the channel dimension of HSI into three without losing the essential color of elastic fibers. The set of this function includes Linear Discriminant Function (LDF) and the transmittance spectrum of Eosin and Hematoxylin which has proved to best preserve the underlying important features of EVG stained image while reducing the dimensionality of hyperspectral H&E. The experimental result proves the feasibility of our proposed method to generate realistic EVG stained image from its corresponding H&E stained one.
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