Cellular resolution of optical coherence tomography (OCT) is vital to achieving precise diagnosis by offering high-quality images of slide-free histology. Currently, the common solution is to apply dynamic focusing to axially translate the focus through the region of interest with a high numerical aperture (N.A.) objective, followed by Z-stacking to rebuild a high-resolution 3D volume. To accelerate the imaging acquisition, this work developed optical metasurface plates to generate multiple foci along the axial direction. Two-/three-/seven-foci had been testified with bead phantom using a scanning OCT. Human skin and human brain samples were imaged with cellular resolution.
In this work, we developed a new method for high throughput and high content spectral imaging flow cytometry based on structured linear spot array excitation. This method leverages equally spaced laser spots for illumination, scanning of a single cell with cell movement, and the cell image is reconstructed by splitting and assembling PMT signals. To demonstrate this method, we first built an imaging flow cytometer with dual-laser and five imaging channels (Bright-field, FITC, PE, PI, APC). More specially, due to the excellent scalability of this method, for the first time, we demonstrated a high-throughput hyperspectral imaging flow cytometer by integrating a high speed 32-channel spectrometer. This system obtains 32 spectral images of 1 μm resolution at the cell flowing speed of 5 m/s with the maximum throughput up to 5000 eps.
Flow cytometer is a powerful tool for the quantitative analysis of a large population of cells. A key factor determining the measurement accuracy and system stability is the illumination beam. The conventional beam shaper is composed of multiple geometrical optical elements, which should be precisely calibrated to produce elliptical Gaussian beams. It is difficult to control the sizes and positions of the focal spots formed by different wavelengths. A new beam shaping method based on diffractive optical element (DOE) is developed for rectangular flattop spots with multi-wavelength illumination. Its benefits include high simplicity, design flexibility, uniform illumination, and multifunctionality.
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