Optical coherence tomography (OCT) is an imaging technology which can be used to obtain the high resolution cross sectional image of living biological tissues. It has been used to evaluate the structure and function of animal and human kidneys. Preliminary animal and human data suggest that OCT imaging might be a useful non-invasive tool for characterizing renal tubular lumens, such as the opening status of tubular lumens. In this pilot study, living animal kidneys (dog, rat and mouse) were imaged using a swept source OCT (SS OCT) or spectral domain OCT(SD OCT). In vivo imaging scans were carried out using an OCT microscope setup (5×) and by placing the imaging probe above the surface of the living kidney. Semi-quantitative analysis of the OCT images was performed to evaluate the density of the kidney tubules on the surface layer of the cortex. In addition, histological images of the kidneys were restructured to form nephron three-dimensional structure for comparison with the 3D OCT imaging. This study suggests that quantitative OCT imaging might be useful for visualizing the fine structure of the living kidney and determining the density of renal tubules.
Optical coherence tomography (OCT) is a useful optical biopsy tool. Its potential in the evaluation of living kidney has been demonstrated. One of such applications is to predict the acute tubular necrosis (ATN) associated with kidney transplantation. The light dense and lucent regions seen in 2D OCT scanning are considered as a useful marker of the renal tubules. In this study, the OCT examination of living human kidney was carried out using a swept source (SS) OCT (SS-OCT) system. The light lucent regions in the cortex obtained on the OCT scan were defined as low signal cavities. The structure features of characteristic cavities in 2D and simulated 3D OCT images were quantitatively analyzed using Amira and Matlab programs. Although the imaging acquisition and real-time analysis were feasible for the examination of donor kidney before and after the transplantation, as the imaging acquisition was obtained under the hand-hold fashion, OCT images might become blurred and the tubules became hardly distinguishable from cortex background, especially for 3D images. In order to optimize the scanning parameters of the OCT imaging process, the influence of the jittering of the living kidney on the quality of OCT imaging and the distortion of the renal tubule structure were studied.
Photodynamic therapy (PDT) utilizes the combined action of photosensitizer, light and molecular oxygen to generate singlet oxygen to treat various diseases. Photosensitizer is a key component and its optical properties and singlet oxygen quantum yield determine the effectiveness of its photodynamic reaction. This study evaluated the singlet oxygen production of a novel water solubility chlorin photosensitizer - namely YLG-I, which was developed in China. The absorption spectra and singlet oxygen luminescence different solvents were evaluated. It was estimated that the singlet oxygen quantum yield of the new photosensitizer YLG-I was at the same level of the potent chlorin sensitizer mTHPC. Preliminary evaluation suggested that YLG-I was a promising photosensitizer for PDT. This presentation will report the detection of singlet oxygen luminescence of the newly developed PDT sensitizer.
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