Tumor invasion to the peritoneum is a poor prognostic factor in cancer patients. Accurate diagnosis of disseminated
peritoneal tumors is essential to accurate cancer staging. To date, peritoneal washing cytology during laparotomy has
been used for diagnosis of peritoneal dissemination of gastrointestinal cancer, but its sensitivity has not been satisfactory.
Thus, a more direct approach is indispensable to detect peritoneal dissemination in vivo. Fluorescein diacrylate (FDAcr)
is an esterase-sensitive fluorescent probe derived from fluorescein. In cancer cells, fluorescent fluorescein generated by
exogenous application of FDAcr selectively deposits owing to its stronger hydrolytic enzyme activity and its lower
leakage rate. We examined whether FDAcr can specifically detect disseminated peritoneal tumors in athymic nude
mouse models. Intraperitoneally administered FDAcr revealed disseminated peritoneal microscopic tumors not readily
recognized on white-light imaging. These results suggest that FDAcr is a useful probe for detecting disseminated
peritoneal tumors.
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