Despite the effectiveness of using fractional picosecond 1064-nm Nd:YAG lasers for facial rejuvenation or resurfacing, the underlying mechanisms of the dermal remodeling with this nonablative treatment are not fully understood. We perform harmonic generation and reflectance confocal microscopy for the in vivo observation of laser-induced optical breakdown. We have found that the induced vacuoles extend to the papillary dermis using second harmonic generation imaging, which is different from the histopathology examinations and previous studies using optical coherence tomography and reflectance confocal microscopy. Our finding suggests that the fractional picosecond laser may induce collagen degeneration through suboptimal thermal effects.
We optimized the spectral coverage of self-phase-modulation-enabled femtosecond fiber sources by careful investigations into the influence of input pulse width, fiber length, and fiber damage, and we have demonstrated a widely tunable source ranging from 740-1250 nm for two-photon microscopy applications. In addition, tens of milliwatt tunable near-UV/visible spectrum is easily obtained by a frequency-doubled conversion, and the gap between the fundamental and frequency-doubled spectra can be filled with the nonlinear wave breaking around 650 nm. A multi-modality microscopy incorporating two-photon microscopy and confocal fluorescence microscopy was also demonstrated to prove the versatility of our development for biomedical imaging.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.