Mr. Hui Zeng Profile

Hui Zeng

at Tsinghua Univ.

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Proceedings Article | 18 December 2019 Paper

Dual-color quantum dot structured illumination microscopy using single LED

Hui Zeng, Huaidong Yang

Proceedings Volume 11337, 113370T (2019) https://doi.org/10.1117/12.2545456

KEYWORDS: Microscopy, Microscopes, Quantum dots, Digital micromirror devices, Fluorescent markers, Super resolution

Read Abstract +

Structured illumination microscopy (SIM) is a kind of wide-field super-resolution (SR) imaging technique which can double the resolution than convenient microscope. Because it doesn’t need any special fluorescent labels and high illumination intensity, it’s an ideal technique for high frame multicolor subcellular imaging in large field of view. Nevertheless, most of the previous multicolor SIM schemes use multiple excitation light to excite different fluorescence labels sequentially, not only making the system complex, but also limiting the ability to observe dynamic interaction between subcellular structures. Quantum dot (QD) is a kind of novel fluorescence label which has a wide absorption spectrum. Utilizing this special property of QD, we present a simple solution of multicolor SIM. The solution uses only a single LED as excitation light source and keep the ability to simultaneous image different colors. To demonstrate the capabilities of our system, we imaged the Blue-ray disc labeled by dual-color QDs.

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