Fluorescence diffuse tomography for tumor detection and monitoring

Irina V. Balalaeva; Anna G. Orlova; Marina V. Shirmanova; Elena A. Kibraeva; Elena V. Zagainova; Ilya V. Turchin

doi:10.1117/12.740615

7 May 2007 Fluorescence diffuse tomography for tumor detection and monitoring

Irina V. Balalaeva, Anna G. Orlova, Marina V. Shirmanova, Elena A. Kibraeva, Elena V. Zagainova, Ilya V. Turchin

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 653509 (2007) https://doi.org/10.1117/12.740615
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

Strong light scattering and absorption limit visualization of the internal structure of biological tissue. Only special tools for turbid media imaging, such as optical diffuse tomography, enable noninvasive investigation of the internal biological tissues, including visualization and intravital monitoring of deep tumors. In this work the preliminary results of fluorescence diffuse tomography (FDT) of small animals are presented. Using of exogenous fluorophores, targeted specifically at tumor cells, and fluorescent proteins expressed endogenously can significantly increase the contrast of obtained images. Fluorescent compounds of different nature, such as sulphonated aluminium phthalocyanine (Photosens), red fluorescing proteins and CdTe/CdSe-core/shell nanocrystals (quantum dots) were applied. The animal was scanned in the transilluminative configuration by low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at the wavelength of 532 nm or semiconductor laser at the wavelength of 655 nm. Photosens was injected intravenously into linear mice with metastazing Lewis lung carcinoma in dose 4 mg/kg. Quantum dots (5x10^-11 M) or protein DsRed2 (1-5x10^-6 M) in glass capsules (inner diameter 2-3 mm) were placed inside the esophagus of 7-day-old hairless rats (18-20 g) to simulate marked tumors. Cells of HEK-293 Phoenix line, transitory transfected with Turbo-RFP protein gene, were injected hypodermically to immunodeficient mice. This work demonstrates potential capabilities of FDT method for detection and monitoring of deep fluorescent-labeled tumors in animal models. Strong advantages of fluorescent proteins and quantum dots over the traditional photosensitizer for FDT imaging are shown.

Citation Download Citation

Irina V. Balalaeva, Anna G. Orlova, Marina V. Shirmanova, Elena A. Kibraeva, Elena V. Zagainova, and Ilya V. Turchin "Fluorescence diffuse tomography for tumor detection and monitoring", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 653509 (7 May 2007); https://doi.org/10.1117/12.740615

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KEYWORDS

Tumors

Luminescence

Quantum dots

Fluorescent proteins

Proteins

Tissues

Tomography

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