Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)

Chetan A. Patil; Jeroen Kalkman; Dirk Faber; John S. Penn; Ton G. van Leeuwen; Anita Mahadevan-Jansen

doi:10.1117/12.843106

2 March 2010 Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)

Chetan A. Patil, Jeroen Kalkman, Dirk Faber, John S. Penn, Ton G. van Leeuwen, Anita Mahadevan-Jansen

Author Affiliations +

Proceedings Volume 7550, Ophthalmic Technologies XX; 75501B (2010) https://doi.org/10.1117/12.843106
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Raman spectroscopy (RS) and Optical Coherence Tomography (OCT) are powerful tools for optical analysis of tissues with complimentary strengths. OCT excels and visualizing tissue microstructure while RS can relay tissue biochemical composition. Both instruments have significant potential to serve as valuable tools in non-invasive characterization of the rodent models of retinal disease. In this abstract we present the design and application of a common detector combined RS-OCT instrument for evaluating the morphological and biochemical differences in a rat model for oxygen induced retinopathy. Rat pups that have undergone a variable oxygen treatment are compared to rats raised in room air. Images and spectra collected at an age of 26 days postnatal demonstrate differences in both the thickness of the inner and outer nuclear layers, but also in the biochemical composition.

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Chetan A. Patil, Jeroen Kalkman, Dirk Faber, John S. Penn, Ton G. van Leeuwen, and Anita Mahadevan-Jansen "Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)", Proc. SPIE 7550, Ophthalmic Technologies XX, 75501B (2 March 2010); https://doi.org/10.1117/12.843106

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